Auxin regulates SNARE-dependent vacuolar morphology restricting cell size

The control of cellular growth is central to multicellular patterning. In plants, the encapsulating cell wall literally binds neighbouring cells to each other and limits cellular sliding/migration. In contrast to its developmental importance, growth regulation is poorly understood in plants. Here, w...

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Bibliographic Details
Published in:eLife 2015-03, Vol.4
Main Authors: Löfke, Christian, Dünser, Kai, Scheuring, David, Kleine-Vehn, Jürgen
Format: Article
Language:English
Subjects:
Arabidopsis - cytology
Arabidopsis - genetics
Arabidopsis - metabolism
Arabidopsis Proteins - genetics
Arabidopsis Proteins - metabolism
auxin
Cell Biology
Cell size
Cell Size - drug effects
Cell walls
Cytology
Experiments
F-Box Proteins - genetics
F-Box Proteins - metabolism
growth
Growth rate
Immunoblotting
Indoleacetic Acids - metabolism
Indoleacetic Acids - pharmacology
Luminescent Proteins - genetics
Luminescent Proteins - metabolism
Microscopy, Confocal
Morphogenesis
Morphology
Pattern formation
Plant Biology
Plant Growth Regulators - metabolism
Plant Growth Regulators - pharmacology
Plants, Genetically Modified
Proteins
Qb-SNARE Proteins - genetics
Qb-SNARE Proteins - metabolism
Receptors, Cell Surface - genetics
Receptors, Cell Surface - metabolism
SNAP receptors
SNARE Proteins - genetics
SNARE Proteins - metabolism
Transcription factors
vacuole
Vacuoles - drug effects
Vacuoles - metabolism
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Summary:The control of cellular growth is central to multicellular patterning. In plants, the encapsulating cell wall literally binds neighbouring cells to each other and limits cellular sliding/migration. In contrast to its developmental importance, growth regulation is poorly understood in plants. Here, we reveal that the phytohormone auxin impacts on the shape of the biggest plant organelle, the vacuole. TIR1/AFBs-dependent auxin signalling posttranslationally controls the protein abundance of vacuolar SNARE components. Genetic and pharmacological interference with the auxin effect on vacuolar SNAREs interrelates with auxin-resistant vacuolar morphogenesis and cell size regulation. Vacuolar SNARE VTI11 is strictly required for auxin-reliant vacuolar morphogenesis and loss of function renders cells largely insensitive to auxin-dependent growth inhibition. Our data suggests that the adaptation of SNARE-dependent vacuolar morphogenesis allows auxin to limit cellular expansion, contributing to root organ growth rates.
ISSN:2050-084X
2050-084X
DOI:10.7554/eLife.05868