Exploring Macrophage-Dependent Wound Regeneration During Mycobacterial Infection in Zebrafish

The molecular and cellular mechanisms associated with tissue degradation or regeneration in an infectious context are poorly defined. Herein, we explored the role of macrophages in orchestrating either tissue regeneration or degradation in zebrafish embryos pre-infected with the fish pathogen . Zebr...

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Bibliographic Details
Published in:Frontiers in immunology 2022-03, Vol.13, p.838425-838425
Main Authors: Bohaud, Candice, Johansen, Matt D, Varga, Béla, Contreras-Lopez, Rafael, Barthelaix, Audrey, Hamela, Claire, Sapède, Dora, Cloitre, Thierry, Gergely, Csilla, Jorgensen, Christian, Kremer, Laurent, Djouad, Farida
Format: Article
Language:English
Subjects:
Animals
Immunology
infection
Innate immunity
Life Sciences
Macrophages - metabolism
macrophages sub-types
Mycobacterium Infections
Mycobacterium marinum
necrosis
regeneration
zebrafish
Zebrafish - metabolism
Zebrafish Proteins - metabolism
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Summary:The molecular and cellular mechanisms associated with tissue degradation or regeneration in an infectious context are poorly defined. Herein, we explored the role of macrophages in orchestrating either tissue regeneration or degradation in zebrafish embryos pre-infected with the fish pathogen . Zebrafish were inoculated with different infectious doses of prior to fin resection. While mild infection accelerated fin regeneration, moderate or severe infection delayed this process by reducing blastemal cell proliferation and impeding tissue morphogenesis. This was correlated with impaired macrophage recruitment at the wound of the larvae receiving high infectious doses. Macrophage activation characterized, in part, by a high expression level of was exacerbated in severely infected fish during the early phase of the regeneration process, leading to macrophage necrosis and their complete absence in the later phase. Our results demonstrate how a mycobacterial infection influences the macrophage response and tissue regenerative processes.
ISSN:1664-3224
1664-3224
DOI:10.3389/fimmu.2022.838425