Phytochemical Profile, Antioxidant Activity, Anti-Hyperglycemic Effect and Toxicity Assessment of Ridolfia segetum (L.) Moris Extract

• Published in: Life (Basel, Switzerland) Switzerland), 2022-12, Vol.13 (1), p.44
• Main Authors: El Karkouri, Jamila, Kchibale, Amale, Chroho, Mounia, Eddamsyry, Brahim, Touijer, Hanane, El Makhoukhi, Fadoua, Handaq, Nadia, Eto, Bruno, Salamatullah, Ahmad Mohammad, Bourhia, Mohammed, Zair, Touriya
• Format: Article
• Language: English
• Subjects: Acetic acid; Acute toxicity; antihyperglycemic; Antioxidants; Biocompatibility; Catechin; Catechol; Chemical composition; Constipation; Coumarin; Diabetes; DPPH; Ethyl acetate; Flavonoids; FRAP; Gallic acid; Gas flow; Glucose; Glucose tolerance; Herbal medicine; High performance liquid chromatography; Hyperglycemia; In vivo methods and tests; Liquid chromatography; Naringenin; Phenolic compounds; Phenols; Phytochemicals; Polyphenols; Ridolfia segetum (L.) Moris; Rosmarinic acid; Scientific imaging; Tannic acid; toxicity; Toxicity testing; Trace elements; Vanillic acid; α-Amylase
• ISSN: 2075-1729; 2075-1729
• DOI: 10.3390/life13010044

The present work was designed to study the chemical composition, antioxidant, antihyperglycemic effect, and toxicity assessment of (L.) Moris extract. The chemical composition was studied by use of high-performance liquid chromatography (HPLC). Antioxidant power was tested by use of DPPH and FRAP assays. The antihyperglycemic effect was tested by use of a glucose tolerance test, while toxicity assessment was done in vivo by use of Wistar rats for 14 days. Analysis of the extract by HPLC-UV revealed the presence of gallic acid, catechol, vanillic acid, catechin, tannic acid, rosmarinic acid, naringenin, and coumarin acid. The crude hydroethanolic extract possessed high levels of total phenols (15.6 ± 1.76 mg EAG/g), condensed tannins (383.49 mg ECat/g DM), and flavonoid (11.63 mg EQ/g). The findings showed that the studied extract possessed good antioxidant power with IC values equal to 550, 650, 700 µg/mL respectively for the decoction, the ethyl acetate fraction (F ), and the ethyl acetate fraction (F ). For the antioxidant activity by FRAP, the aqueous fraction (F ) and the aqueous extract (F ) showed CE values of 0.33 mg/mL and 0.4 mg/mL, respectively. Glucose tolerance test analysis showed that (L.) Moris decoction had a significant postprandial antihyperglycemic effect in normal Wistar rats. The results of the acute toxicity test showed that the decoction was not toxic even at 2 g/Kg. Pancreatic α-amylase activity was significantly inhibited in the presence of (L.) Moris extract (IC = 0.133 ± 0.09 mg/mL). The outcome of the present work showed that (L.) Moris is very rich in phenolic compounds with potent antioxidant and antihyperglycemic effects.