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Validation of Fucoxanthin from Microalgae Phaeodactylum tricornutum for the Detection of Amyloid Burden in Transgenic Mouse Models of Alzheimer’s Disease
The visualization of misfolded Aβ peptides by using fluorescence chemical dyes is very important in Alzheimer’s disease (AD) diagnosis. Here, we describe the fluorescent substance, fucoxanthin, which detects Aβ aggregates in the brain of AD transgenic mouse models. We found that fucoxanthin from the...
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Published in: | Applied sciences 2021-07, Vol.11 (13), p.5878 |
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description | The visualization of misfolded Aβ peptides by using fluorescence chemical dyes is very important in Alzheimer’s disease (AD) diagnosis. Here, we describe the fluorescent substance, fucoxanthin, which detects Aβ aggregates in the brain of AD transgenic mouse models. We found that fucoxanthin from the microalgae Phaeodactylum tricornutum has fluorescent excitation and emission wavelengths without any interference for Aβ interaction. Thus, we applied it to monitor Aβ aggregation in AD transgenic mouse models. Aβ plaques were visualized using fucoxanthin in the brain tissue of APP/PS1 and 5×FAD mice by histological staining with different staining methods. By comparing fucoxanthin-positive and thioflavin S-positive stained regions in the brains, we found that they are colocalized and that fucoxanthin can detect Aβ aggregates. Our finding suggests that fucoxanthin from P. tricornutum can be a new Aβ fluorescent imaging reagent in AD diagnosis. |
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Here, we describe the fluorescent substance, fucoxanthin, which detects Aβ aggregates in the brain of AD transgenic mouse models. We found that fucoxanthin from the microalgae Phaeodactylum tricornutum has fluorescent excitation and emission wavelengths without any interference for Aβ interaction. Thus, we applied it to monitor Aβ aggregation in AD transgenic mouse models. Aβ plaques were visualized using fucoxanthin in the brain tissue of APP/PS1 and 5×FAD mice by histological staining with different staining methods. By comparing fucoxanthin-positive and thioflavin S-positive stained regions in the brains, we found that they are colocalized and that fucoxanthin can detect Aβ aggregates. Our finding suggests that fucoxanthin from P. tricornutum can be a new Aβ fluorescent imaging reagent in AD diagnosis.</description><identifier>ISSN: 2076-3417</identifier><identifier>EISSN: 2076-3417</identifier><identifier>DOI: 10.3390/app11135878</identifier><language>eng</language><publisher>Basel: MDPI AG</publisher><subject>AD diagnosis ; Aggregates ; Algae ; Alzheimer's disease ; amyloid plaque ; Animal cognition ; Animal models ; Aquatic microorganisms ; Diagnosis ; Dyes ; Ethanol ; Fluorescence ; fluorescent dye ; Fluoroscopic imaging ; Fucoxanthin ; Laboratory animals ; Mutation ; Neuroimaging ; Oxidative stress ; Peptides ; Phaeodactylum tricornutum ; Plaques ; Presenilin 1 ; Reagents ; Rodents ; Staining ; Transgenic mice ; Visualization ; Wavelengths</subject><ispartof>Applied sciences, 2021-07, Vol.11 (13), p.5878</ispartof><rights>2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c364t-800715157063591909895a590c8840a1d0dfa2393efe04cc04b67b02c297db1f3</citedby><cites>FETCH-LOGICAL-c364t-800715157063591909895a590c8840a1d0dfa2393efe04cc04b67b02c297db1f3</cites><orcidid>0000-0002-9121-7031 ; 0000-0002-6880-8861 ; 0000-0002-0786-1482</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/2549263622/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/2549263622?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>314,780,784,25753,27924,27925,37012,44590,75126</link.rule.ids></links><search><creatorcontrib>Lee, A-Hyeon</creatorcontrib><creatorcontrib>Hong, Sung-Chul</creatorcontrib><creatorcontrib>Park, Inwook</creatorcontrib><creatorcontrib>Yoon, Soljee</creatorcontrib><creatorcontrib>Kim, YoungSoo</creatorcontrib><creatorcontrib>Kim, Jinsik</creatorcontrib><creatorcontrib>Yang, Seung-Hoon</creatorcontrib><title>Validation of Fucoxanthin from Microalgae Phaeodactylum tricornutum for the Detection of Amyloid Burden in Transgenic Mouse Models of Alzheimer’s Disease</title><title>Applied sciences</title><description>The visualization of misfolded Aβ peptides by using fluorescence chemical dyes is very important in Alzheimer’s disease (AD) diagnosis. Here, we describe the fluorescent substance, fucoxanthin, which detects Aβ aggregates in the brain of AD transgenic mouse models. We found that fucoxanthin from the microalgae Phaeodactylum tricornutum has fluorescent excitation and emission wavelengths without any interference for Aβ interaction. Thus, we applied it to monitor Aβ aggregation in AD transgenic mouse models. Aβ plaques were visualized using fucoxanthin in the brain tissue of APP/PS1 and 5×FAD mice by histological staining with different staining methods. By comparing fucoxanthin-positive and thioflavin S-positive stained regions in the brains, we found that they are colocalized and that fucoxanthin can detect Aβ aggregates. Our finding suggests that fucoxanthin from P. tricornutum can be a new Aβ fluorescent imaging reagent in AD diagnosis.</description><subject>AD diagnosis</subject><subject>Aggregates</subject><subject>Algae</subject><subject>Alzheimer's disease</subject><subject>amyloid plaque</subject><subject>Animal cognition</subject><subject>Animal models</subject><subject>Aquatic microorganisms</subject><subject>Diagnosis</subject><subject>Dyes</subject><subject>Ethanol</subject><subject>Fluorescence</subject><subject>fluorescent dye</subject><subject>Fluoroscopic imaging</subject><subject>Fucoxanthin</subject><subject>Laboratory animals</subject><subject>Mutation</subject><subject>Neuroimaging</subject><subject>Oxidative stress</subject><subject>Peptides</subject><subject>Phaeodactylum tricornutum</subject><subject>Plaques</subject><subject>Presenilin 1</subject><subject>Reagents</subject><subject>Rodents</subject><subject>Staining</subject><subject>Transgenic mice</subject><subject>Visualization</subject><subject>Wavelengths</subject><issn>2076-3417</issn><issn>2076-3417</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>PIMPY</sourceid><sourceid>DOA</sourceid><recordid>eNpNkc9uFDEMxkcIJKrSEy8QiSNayN-ZybG0tFRqBYe218iTOLtZzUyWJCOxnPoaiLfjSQhdQPXB_mR9-tmym-Y1o--E0PQ97HaMMaH6rn_WHHHatSshWff8iX7ZnOS8pTU0Ez2jR83PexiDgxLiTKInF4uN32AumzATn-JEboJNEcY1IPmyAYwObNmPy0RKCjameSlV-5hI2SA5x4L2H-p02o8xOPJhSQ5nUoG3Cea8xjlYchOXjDU7HPOjefy-wTBh-vXwI5PzkBEyvmpeeBgznvytx83dxcfbs0-r68-XV2en1ysrWllWPaUdU0x1tBVKM011rxUoTW3fSwrMUeeBCy3QI5XWUjm03UC55bpzA_PiuLk6cF2ErdmlMEHamwjBPDZiWhtIJdgRDfOU1-Nhx1UvmbRaC8GV4zgMzklnK-vNgbVL8euCuZhtXNJc1zdcSc1b0XJeXW8PrnrcnBP6_1MZNX-eaZ48U_wGz9eTlQ</recordid><startdate>20210701</startdate><enddate>20210701</enddate><creator>Lee, A-Hyeon</creator><creator>Hong, Sung-Chul</creator><creator>Park, Inwook</creator><creator>Yoon, Soljee</creator><creator>Kim, YoungSoo</creator><creator>Kim, Jinsik</creator><creator>Yang, Seung-Hoon</creator><general>MDPI AG</general><scope>AAYXX</scope><scope>CITATION</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0002-9121-7031</orcidid><orcidid>https://orcid.org/0000-0002-6880-8861</orcidid><orcidid>https://orcid.org/0000-0002-0786-1482</orcidid></search><sort><creationdate>20210701</creationdate><title>Validation of Fucoxanthin from Microalgae Phaeodactylum tricornutum for the Detection of Amyloid Burden in Transgenic Mouse Models of Alzheimer’s Disease</title><author>Lee, A-Hyeon ; Hong, Sung-Chul ; Park, Inwook ; Yoon, Soljee ; Kim, YoungSoo ; Kim, Jinsik ; Yang, Seung-Hoon</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c364t-800715157063591909895a590c8840a1d0dfa2393efe04cc04b67b02c297db1f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>AD diagnosis</topic><topic>Aggregates</topic><topic>Algae</topic><topic>Alzheimer's disease</topic><topic>amyloid plaque</topic><topic>Animal cognition</topic><topic>Animal models</topic><topic>Aquatic microorganisms</topic><topic>Diagnosis</topic><topic>Dyes</topic><topic>Ethanol</topic><topic>Fluorescence</topic><topic>fluorescent dye</topic><topic>Fluoroscopic imaging</topic><topic>Fucoxanthin</topic><topic>Laboratory animals</topic><topic>Mutation</topic><topic>Neuroimaging</topic><topic>Oxidative stress</topic><topic>Peptides</topic><topic>Phaeodactylum tricornutum</topic><topic>Plaques</topic><topic>Presenilin 1</topic><topic>Reagents</topic><topic>Rodents</topic><topic>Staining</topic><topic>Transgenic mice</topic><topic>Visualization</topic><topic>Wavelengths</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lee, A-Hyeon</creatorcontrib><creatorcontrib>Hong, Sung-Chul</creatorcontrib><creatorcontrib>Park, Inwook</creatorcontrib><creatorcontrib>Yoon, Soljee</creatorcontrib><creatorcontrib>Kim, YoungSoo</creatorcontrib><creatorcontrib>Kim, Jinsik</creatorcontrib><creatorcontrib>Yang, Seung-Hoon</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Directory of Open Access Journals</collection><jtitle>Applied sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lee, A-Hyeon</au><au>Hong, Sung-Chul</au><au>Park, Inwook</au><au>Yoon, Soljee</au><au>Kim, YoungSoo</au><au>Kim, Jinsik</au><au>Yang, Seung-Hoon</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Validation of Fucoxanthin from Microalgae Phaeodactylum tricornutum for the Detection of Amyloid Burden in Transgenic Mouse Models of Alzheimer’s Disease</atitle><jtitle>Applied sciences</jtitle><date>2021-07-01</date><risdate>2021</risdate><volume>11</volume><issue>13</issue><spage>5878</spage><pages>5878-</pages><issn>2076-3417</issn><eissn>2076-3417</eissn><abstract>The visualization of misfolded Aβ peptides by using fluorescence chemical dyes is very important in Alzheimer’s disease (AD) diagnosis. 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subjects | AD diagnosis Aggregates Algae Alzheimer's disease amyloid plaque Animal cognition Animal models Aquatic microorganisms Diagnosis Dyes Ethanol Fluorescence fluorescent dye Fluoroscopic imaging Fucoxanthin Laboratory animals Mutation Neuroimaging Oxidative stress Peptides Phaeodactylum tricornutum Plaques Presenilin 1 Reagents Rodents Staining Transgenic mice Visualization Wavelengths |
title | Validation of Fucoxanthin from Microalgae Phaeodactylum tricornutum for the Detection of Amyloid Burden in Transgenic Mouse Models of Alzheimer’s Disease |
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