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Validation of Fucoxanthin from Microalgae Phaeodactylum tricornutum for the Detection of Amyloid Burden in Transgenic Mouse Models of Alzheimer’s Disease

The visualization of misfolded Aβ peptides by using fluorescence chemical dyes is very important in Alzheimer’s disease (AD) diagnosis. Here, we describe the fluorescent substance, fucoxanthin, which detects Aβ aggregates in the brain of AD transgenic mouse models. We found that fucoxanthin from the...

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Published in:Applied sciences 2021-07, Vol.11 (13), p.5878
Main Authors: Lee, A-Hyeon, Hong, Sung-Chul, Park, Inwook, Yoon, Soljee, Kim, YoungSoo, Kim, Jinsik, Yang, Seung-Hoon
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description The visualization of misfolded Aβ peptides by using fluorescence chemical dyes is very important in Alzheimer’s disease (AD) diagnosis. Here, we describe the fluorescent substance, fucoxanthin, which detects Aβ aggregates in the brain of AD transgenic mouse models. We found that fucoxanthin from the microalgae Phaeodactylum tricornutum has fluorescent excitation and emission wavelengths without any interference for Aβ interaction. Thus, we applied it to monitor Aβ aggregation in AD transgenic mouse models. Aβ plaques were visualized using fucoxanthin in the brain tissue of APP/PS1 and 5×FAD mice by histological staining with different staining methods. By comparing fucoxanthin-positive and thioflavin S-positive stained regions in the brains, we found that they are colocalized and that fucoxanthin can detect Aβ aggregates. Our finding suggests that fucoxanthin from P. tricornutum can be a new Aβ fluorescent imaging reagent in AD diagnosis.
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subjects AD diagnosis
Aggregates
Algae
Alzheimer's disease
amyloid plaque
Animal cognition
Animal models
Aquatic microorganisms
Diagnosis
Dyes
Ethanol
Fluorescence
fluorescent dye
Fluoroscopic imaging
Fucoxanthin
Laboratory animals
Mutation
Neuroimaging
Oxidative stress
Peptides
Phaeodactylum tricornutum
Plaques
Presenilin 1
Reagents
Rodents
Staining
Transgenic mice
Visualization
Wavelengths
title Validation of Fucoxanthin from Microalgae Phaeodactylum tricornutum for the Detection of Amyloid Burden in Transgenic Mouse Models of Alzheimer’s Disease
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