Orientation of Antigen Display on Self-Assembling Protein Nanoparticles Influences Immunogenicity

Self-assembling protein nanoparticles (SAPN) serve as a repetitive antigen delivery platform with high-density epitope display; however, antigen characteristics such as size and epitope presentation can influence the immunogenicity of the assembled particle and are aspects to consider for a rational...

Full description

Saved in:
Bibliographic Details
Published in:Vaccines (Basel) 2021-01, Vol.9 (2), p.103
Main Authors: Schneider, Cosette G, Taylor, Justin A, Sibilo, Michael Q, Miura, Kazutoyo, Mallory, Katherine L, Mann, Christopher, Karch, Christopher, Beck, Zoltan, Matyas, Gary R, Long, Carole A, Bergmann-Leitner, Elke, Burkhard, Peter, Angov, Evelina
Format: Article
Language:English
Subjects:
Amino acids
Antibodies
Antigen presentation
Antigens
Autoantigens
Cloning
display
E coli
Epitopes
Folding
Immune response
Immune system
Immunogenicity
Malaria
Merozoite surface protein 1
multi-stage
Nanoparticles
Parasites
PfCelTOS
PfMSP119
Proteins
Rabbits
self-assembling protein nanoparticles
Self-assembly
Sporozoites
vaccine
Vaccines
Zygotes
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Self-assembling protein nanoparticles (SAPN) serve as a repetitive antigen delivery platform with high-density epitope display; however, antigen characteristics such as size and epitope presentation can influence the immunogenicity of the assembled particle and are aspects to consider for a rationally designed effective vaccine. Here, we characterize the folding and immunogenicity of heterogeneous antigen display by integrating (a) dual-stage antigen SAPN presenting the ( ) merozoite surface protein 1 subunit, PfMSP1 , and cell-traversal protein for ookinetes and sporozoites, PfCelTOS, in addition to (b) a homogenous antigen SAPN displaying two copies of PfCelTOS. Mice and rabbits were utilized to evaluate antigen-specific humoral and cellular induction as well as functional antibodies via growth inhibition of the blood-stage parasite. We demonstrate that antigen orientation and folding influence the elicited immune response, and when appropriately designed, SAPN can serve as an adaptable platform for an effective multi-antigen display.
ISSN:2076-393X
2076-393X
DOI:10.3390/vaccines9020103