Overexpressed cold inducible RNA-binding protein improves cell viability and EGF expression in glial cells

• Published in: BMC cell biology 2022-12, Vol.23 (1), p.58-58, Article 58
• Main Authors: Liu, Aijun, Li, Shouchun, Jiao, Yongcheng, Kong, Haibo, Zhang, Zhiwen
• Format: Article
• Language: English
• Subjects: Antibodies; Apoptosis; Bcl-2 protein; Binding proteins; Brain; Brain-derived neurotrophic factor; Cell Survival; Cell viability; Cold; Cold-inducibleRNA-binding protein; Efficiency; Epidermal growth factor; Epidermal Growth Factor - metabolism; Gene expression; Glial cells; Glucose; Health aspects; Hypothermia, Induced; Hypoxia; Infections; Injuries; Messenger RNA; Methods; Nerve growth factor; Neuroglia; Neuroglia - metabolism; Neuronal-glial interactions; Neuroprotection; Neuroprotective Agents; Neurotoxicity; Physiological aspects; Proteins; RNA, Messenger - genetics; RNA-binding protein; RNA-Binding Proteins - genetics; RNA-Binding Proteins - metabolism; Statistical analysis; Vascular endothelial growth factor; Vascular Endothelial Growth Factor A
• ISSN: 2661-8850; 2661-8850; 1471-2121
• DOI: 10.1186/s12860-022-00460-3

Cold inducible RNA-binding protein (CIRP) is a key protein in the hypothermic therapy. Highly expressed CIRP exerts a neuroprotective effect on neurons. The aim of this study is to provide the evidence of the protective effects of CIRP on the glial cells and explore the downstream pathway of CIRP. The results of this study demonstrated that the cell viability of the glial cells with CIRP overexpression was increased significantly compared to the control. With CIRP overexpression, the epidermal growth factor (EGF) mRNA expression was found increasing significantly and the mRNA expressions of derived neurotrophic factor (BDNF), bcl-2, vascular endothelial growth factor (VEGF) and nerve growth factor (NGF) were not upregulated compared to the control. EGF and CIRP co-expression was demonstrated on the glial cells. With CIRP expression, EGF expression on the glial cells was increased statistically compared to the control. CIRP overexpression increases the cell viability of the glial cells, exerting a neuroprotective effect. EGF expression is activated on the glial cells with CIRP overexpression, implying a pathway of CIRP neuroprotection via EGF activation.