LPS levels in root canals after the use of ozone gas and high frequency electrical pulses

The present study aims to verify the effect of ozone gas (OZY® System) and high frequency electric pulse (Endox® System) systems on human root canals previously contaminated with Escherichia colilipopolysaccharide (LPS). Fifty single-rooted teeth had their dental crowns removed and root lengths stan...

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Bibliographic Details
Published in:Brazilian oral research 2016, Vol.30 (1)
Main Authors: Melo, Tiago André Fontoura de, Gründling, Grasiela Sabrina Longhi, Montagner, Francisco, Scur, Alcione Luiz, Steier, Liviu, Scarparo, Roberta Kochenborger, Figueiredo, José Antônio Poli de, Vier-Pelisser, Fabiana Vieira
Format: Article
Language:English
Subjects:
Analysis of Variance
Dental Pulp Cavity - microbiology
Dentistry
DENTISTRY, ORAL SURGERY & MEDICINE
Electrocoagulation - methods
Endodontics
Endotoxins
Endotoxins - analysis
Escherichia coli
Humans
Limulus Test
Lipopolysaccharides - analysis
Microbiology
Oxidants, Photochemical
Ozone
Random Allocation
Reproducibility of Results
Root Canal Preparation - methods
Root Canal Therapy
Time Factors
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Summary:The present study aims to verify the effect of ozone gas (OZY® System) and high frequency electric pulse (Endox® System) systems on human root canals previously contaminated with Escherichia colilipopolysaccharide (LPS). Fifty single-rooted teeth had their dental crowns removed and root lengths standardized to 16 mm. The root canals were prepared up to #60 hand K-files and sterilized using gamma radiation with cobalt 60. The specimens were divided into the following five groups (n = 10) based on the disinfection protocol used: OZY® System, one 120-second-pulse (OZY 1p); OZY® System, four 24-second-pulses (OZY 4p); and Endox® System (ENDOX). Contaminated and non-contaminated canals were exposed only to apyrogenic water and used as positive (C+) and negative (C-) controls, respectively. LPS (O55:B55) was administered in all root canals except those belonging to group C-. After performing disinfection, LPS samples were collected from the canals using apyrogenic paper tips. Limulus Amoebocyte Lysate (LAL) was used to quantify the LPS levels, and the data obtained was analyzed using one-way ANOVA. The disinfection protocols used were unable to reduce the LPS levels significantly (p = 0.019). The use of ozone gas and high frequency electric pulses was not effective in eliminating LPS from the root canals.
ISSN:1806-8324
1807-3107
1807-3107
1806-8324
DOI:10.1590/1807-3107BOR-2016.vol30.0019