An improved method for Agrobacterium rhizogenes -mediated transformation of tomato suitable for the study of arbuscular mycorrhizal symbiosis

, an economically important crop grown worldwide, has been used as a model for the study of arbuscular mycorrhizal (AM) symbiosis in non-legume plants for several years and several cDNA array hybridization studies have revealed specific transcriptomic profiles of mycorrhizal tomato roots. However, a...

Full description

Saved in:
Bibliographic Details
Published in:Plant methods 2018-05, Vol.14 (1), p.34-34, Article 34
Main Authors: Ho-Plágaro, Tania, Huertas, Raúl, Tamayo-Navarrete, María I, Ocampo, Juan A, García-Garrido, José M
Format: Article
Language:English
Subjects:
Arbuscular mycorrhiza
Composite plants
Genetic aspects
Genetic research
Genetic transformation
Genotype
Hairy roots
Identification and classification
Methodology
Symbiosis
Tomato
Tomatoes
Transformation
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:, an economically important crop grown worldwide, has been used as a model for the study of arbuscular mycorrhizal (AM) symbiosis in non-legume plants for several years and several cDNA array hybridization studies have revealed specific transcriptomic profiles of mycorrhizal tomato roots. However, a method to easily screen candidate genes which could play an important role during tomato mycorrhization is required. We have developed an optimized procedure for composite tomato plant obtaining achieved through -mediated transformation. This protocol involves the unusual in vitro culture of composite plants between two filter papers placed on the culture media. In addition, we show that is an appropriate molecular marker for the precise selection of cotransformed tomato hairy roots composite plant hairy roots appear to be colonized by the AM fungus in a manner similar to that of normal roots, and a modified construct useful for localizing the expression of promoters putatively associated with mycorrhization was developed and tested. In this study, we present an easy, fast and low-cost procedure to study AM symbiosis in tomato roots.
ISSN:1746-4811
1746-4811
DOI:10.1186/s13007-018-0304-9