Protective Effects of Curcumin against Sodium Arsenite-induced Ovarian Oxidative Injury in a Mouse Model

• Published in: Chinese medical journal 2017-05, Vol.130 (9), p.1026-1032
• Main Authors: Wang, Xiao-Ning, Zhang, Chang-Jun, Diao, Hong-Lu, Zhang, Ying
• Format: Article
• Language: English
• Subjects: Animals; Antioxidant; Curcumin; Mouse; Ovary; Oxidative Stress; p66Shc; Antioxidants; Apoptosis; Arsenic; Arsenites - toxicity; Biomarkers; Cancer; Cellulose; Clinical trials; Curcumin - therapeutic use; Deoxyribonucleic acid; Disease Models, Animal; DNA; DNA methylation; Drug dosages; Embryos; Enzyme-Linked Immunosorbent Assay; Enzymes; Female; Free radicals; Glutathione Peroxidase - metabolism; Hospitals; Immunohistochemistry; Kinases; Laboratory animals; Malondialdehyde - metabolism; Medical research; Medicine; Mice; Original; Ovary - drug effects; Ovary - metabolism; Oxidative stress; Oxidative Stress - drug effects; Physiology; Polycystic Ovary Syndrome - drug therapy; Polycystic Ovary Syndrome - metabolism; Reactive Oxygen Species - metabolism; Reproductive system; Sodium; Sodium Compounds - toxicity; Superoxide Dismutase - metabolism; 亚砷酸钠; 保护作用; 卵巢组织; 姜黄素; 抗氧化损伤; 昆明小鼠; 诱导; 超氧化物歧化酶
• ISSN: 0366-6999; 2542-5641
• DOI: 10.4103/0366-6999.204927

Background： Excessive reactive oxygen species （ROS） may lead to a number of reproductive diseases such as polycystic ovary syndrome. This study aimed to establish an animal model of ovarian oxidative stress and to assess the protective effect ofcurcumin against oxidative injury. Methods： Ovarian oxidative stress was induced in female Kunming mice （n - 40） with intraperitoneal injection of 8 mg/kg sodium arsenite （As） once every other day for 16 days; meanwhile, they were, respectively, treated by intragastric administration of 0, 100, 150, or 200 mg/kg （n - 10/group） curcumin once per day for 21 days. Ten normal mice were used as control. Then, the mice were injected intraperitoneally with BrdU and sacrificed; the right ovaries were collected for hematoxylin and eosin （HE） staining and Brd/J immunohistochemistry, and the left ovaries for enzyme-linked immunosorbent assay （ELISA） and Western blotting analyses. Results： The ELISA results showed that ROS （11.74 ± 0.65 IU/mg in 8 mg/kg AS± 0 mg/kg curcumin group vs. 10.71±0.91 IU/mg in control group, P = 0.021） and malondialdehyde （MDA） （0.32± 0.02 nmol/g in 8 mg/kg AS ＋ 0 mg/kg curcumin group vs. 0.27 ± 0.02 nmolig in control group, P = 0.048） increased while superoxide dismutase （SOD） （3.96 ± 0.36 U/mg in 8 mg/kg AS ＋ 0 mg/kg curcumin group vs. 4.51 ± 0.70 U/rag in control group, P = 0.012） and glutathione peroxidase （17.36 ± 1.63 U/g in 8 mg/kg AS ＋ 0 mg/kg curcumin group vs. 18.92± 1.80 U/g in control group, P - 0.045） decreased in the ovary after injection of As, indicating successful modeling of oxidative stress. Curcumin treatment could considerably increase SOD （4.57 ± 0.68, 4.49 ± 0.27, and 4.56 ± 0.25 U/rag in 100 mg/kg, 150 mg/kg, mad 200 mg/kg curcumin group, respectively, all P 〈 0.05） while significantly reduce ROS （10.64 ±1.38, 10.73 ± 0.71, and 10.67 ± 1.38 IU/mg in 100 mg/kg, 150 mg/kg, and 200 mg/kg curcumin group, respectively, all P 〈 0.05） and M DA （0.28± 0.02, 0.25 ±0.03, and 0.27 ±0.04 nmol/g in 100 mg/kg, 150 mg/kg, and 200 mg/kg curcumin group, respectively; both P〈 0.05） in the ovary. HE staining and BrdU immunohistochemistry of the ovarian tissues indicated the increased amount ofatretic follicles （5.67 ± 0.81,5.84 ± 0.98, and 5.72 ± 0.84 in 100 mg/kg, 150 mg/kg）, and 200 mg/kg curcumin group, respectively, all P 〈 0.05）, and the inhibited proliferation of granular cells under oxidative stress would be reversed by curcumin. Furthermore, the Western blotting of ovarian tissues showed