The Role of Autophagy and Apoptosis in the Combined Action of Plasma-Treated Saline, Doxorubicin, and Medroxyprogesterone Acetate on K562 Myeloid Leukaemia Cells

The anti-cancer properties of plasma-treated solutions (PTS) and their interaction with drugs are one of the most popular topics in modern plasma medicine. Our research involved comparing the effects of four physiological saline solutions (0.9% NaCl, Ringer's solution, Hank's Balanced Salt...

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Published in:International journal of molecular sciences 2023-03, Vol.24 (6), p.5100
Main Authors: Pavlik, Tatyana, Gudkova, Victoria, Razvolyaeva, Darya, Pavlova, Marina, Kostukova, Nadejda, Miloykovich, Lilia, Kolik, Leonid, Konchekov, Evgeny, Shimanovskii, Nikolay
Format: Article
Language:English
Subjects:
Acetates
Acetic acid
Amino acids
Analysis
Antimitotic agents
Antineoplastic agents
Apoptosis
Autophagy
Cancer
Care and treatment
Cold
Cytotoxicity
Doxorubicin
Doxorubicin - pharmacology
Drug interaction
Drugs
Hormones, Sex
Humans
Hydrogen peroxide
K562 Cells
Leukemia
Leukemia, Myeloid
Medroxyprogesterone
Medroxyprogesterone - pharmacology
Medroxyprogesterone acetate
medroxyprogesterone acetate (MPA)
Medroxyprogesterone Acetate - pharmacology
Nitrogen
Oxidation
Physiological aspects
Physiological effects
Physiology
Plasma
plasma-treated solutions (PTS)
Progesterone
Progesterone receptors
Reactive oxygen species
Saline Solution
Saline solutions
Sodium chloride
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Summary:The anti-cancer properties of plasma-treated solutions (PTS) and their interaction with drugs are one of the most popular topics in modern plasma medicine. Our research involved comparing the effects of four physiological saline solutions (0.9% NaCl, Ringer's solution, Hank's Balanced Salt Solution, Hank's Balanced Salt Solution with amino acids added in concentrations observed in the human blood) treated with cold atmospheric plasma and studying the combined cytotoxic effect of PTS with doxorubicin and medroxyprogesterone acetate (MPA). Analysis of the effect of the studied agents on the formation of radicals in the incubation medium, the vitality of K562 myeloid leukaemia cells, and the processes of autophagy and apoptosis in them revealed two key findings. The first is that when using PTS and doxorubicin-containing PTS, autophagy is the predominant process in cancer cells. The second is that combining PTS with MPA enhances apoptotic processes. It was hypothesised that while autophagy is stimulated by the accumulation of reactive oxygen species in the cell, apoptosis is stimulated through specific cell progesterone receptors.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms24065100