Functional interdependence of the actin nucleator Cobl and Cobl-like in dendritic arbor development

Local actin filament formation is indispensable for development of the dendritic arbor of neurons. We show that, surprisingly, the action of single actin filament-promoting factors was insufficient for powering dendritogenesis. Instead, this required the actin nucleator Cobl and its only evolutionar...

Full description

Saved in:
Bibliographic Details
Published in:eLife 2021-07, Vol.10
Main Authors: Izadi, Maryam, Seemann, Eric, Schlobinski, Dirk, Schwintzer, Lukas, Qualmann, Britta, Kessels, Michael M
Format: Article
Language:English
Subjects:
Actin
actin nucleators
Binding sites
Ca2+-steered
Calcium signalling
Calcium-binding protein
Calmodulin
Cell Biology
Cyclin-dependent kinases
dendritic branch initiation sites
F-actin-supported neuronal morphogenesis
F-BAR domain protein syndapin I
functional cooperation of actin nucleators
Kinases
Morphology
Muscle proteins
Neurons
Neuroscience
Proteins
Software
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Local actin filament formation is indispensable for development of the dendritic arbor of neurons. We show that, surprisingly, the action of single actin filament-promoting factors was insufficient for powering dendritogenesis. Instead, this required the actin nucleator Cobl and its only evolutionary distant ancestor Cobl-like acting interdependently. This coordination between Cobl-like and Cobl was achieved by physical linkage by syndapins. Syndapin I formed nanodomains at convex plasma membrane areas at the base of protrusive structures and interacted with three motifs in Cobl-like, one of which was Ca 2+ /calmodulin-regulated. Consistently, syndapin I, Cobl-like’s newly identified N terminal calmodulin-binding site and the single Ca 2+ /calmodulin-responsive syndapin-binding motif all were critical for Cobl-like’s functions. In dendritic arbor development, local Ca 2+ /CaM-controlled actin dynamics thus relies on regulated and physically coordinated interactions of different F-actin formation-promoting factors and only together they have the power to bring about the sophisticated neuronal morphologies required for neuronal network formation in mammals.
ISSN:2050-084X
2050-084X
DOI:10.7554/eLife.67718