Identification and Full-Length Sequence Analysis of a Novel Recombinant Goat Astrovirus Genotype in Guangxi, China

• Published in: Viruses 2024-07, Vol.16 (8), p.1213
• Main Authors: Zhou, Yulu, Xu, Pengju, Huang, Yanhua, Wang, Jie, Cui, Chang, Wang, Yanglin, Luo, Yuhang, Wang, Xiaoling, Xie, Jiang, Li, Fengmei, Wei, Zuzhang, Chen, Ying, Ouyang, Kang, Qin, Yifeng, Pan, Yan, Huang, Weijian
• Format: Article
• Language: English
• Subjects: Amino acid sequence; Amino acids; Animals; Astroviridae; Astroviridae - classification; Astroviridae - genetics; Astroviridae - isolation & purification; Astroviridae Infections - epidemiology; Astroviridae Infections - veterinary; Astroviridae Infections - virology; astroviruses; Capsid protein; Capsid Proteins - genetics; China - epidemiology; Feces; Feces - virology; Genome, Viral; Genomes; Genotype; Genotype & phenotype; Genotypes; goat; Goat Diseases - epidemiology; Goat Diseases - virology; Goats; Goats - virology; Mamastrovirus - classification; Mamastrovirus - genetics; Mamastrovirus - isolation & purification; new strains; Nucleotide sequence; Phylogenetics; Phylogeny; Proteins; recombination; Recombination, Genetic; RNA polymerase; RNA viruses; RNA, Viral - genetics; Sequence analysis; Sequence Analysis, DNA; Sheep; Software; Strains (organisms); Viruses
• ISSN: 1999-4915; 1999-4915
• DOI: 10.3390/v16081213

Astroviruses are single-stranded, positive-sense RNA viruses capable of infecting humans as well as a wide range of mammalian and avian species, with a length of approximately 6.6-7.7 kb. In this study, 139 goat fecal samples collected from the Guangxi province were used for the RT-PCR detection, and two of these were positive for goat astrovirus, with a positivity rate of 1.44% (2/139). The complete genome sequence of an astrovirus strain and the partial genome sequence of a strain astrovirus, named GX WZ 2023 and GX HC 2023, were amplified and sequenced, and their sequence lengths were 6284 nt and 6213 nt, respectively. Among them, the capsid protein of goat astrovirus GX HC 2023 showed the highest amino acid identity of 95.9% with ovine astrovirus GX, which belonged to the MAstV-2 genotype. However, the closest relative of the GX WZ 2023 strain was found to be the caprine astrovirus Sichuan, with a nucleotide sequence identity of 76.8%. The ORF1ab nonstructural protein of this strain showed the highest amino acid identities of 89.2 and 95.8% with the ovine astrovirus S5.1 and caprine astrovirus G5.1 strains, respectively. However, its ORF2 capsid protein has 68.4% amino acid identity with the bovine astrovirus (BAstV) 16 2021 CHN strain and only 21.9-64% amino acid identity with all available strains of goat astrovirus. The GX WZ 2023 strain was recombined with the Chinese (BAstV 16 2021 CHN) and Japanese bovine strains (BAstV JPN 2015) in the ORF2 region. Therefore, the goat astrovirus GX WZ 2023 is proposed as a new member of the family goat astroviridae based on the species classification criteria of the International Committee on Taxonomy of Viruses. These findings enhance our understanding of the prevalence and genetic evolution of goat astrovirus and provide a scientific basis for future studies of these viruses in other animals.