In vitro investigations on interference of selected probiotic candidates with Campylobacter jejuni adhesion and invasion of primary chicken derived cecal and Caco-2 cells

• Published in: Gut pathogens 2024-06, Vol.16 (1), p.30-13, Article 30
• Main Authors: Willer, Thomas, Han, Zifeng, Pielsticker, Colin, Rautenschlein, Silke
• Format: Article
• Language: English
• Subjects: Adenocarcinoma; Analysis; Bacteria; Campylobacter; Campylobacter jejuni; Cecum; Cell culture; Cell interactions; Colonization; Colorectal cancer; Cytokines; Digestive system; E coli; Experiments; Food sources; Foodborne diseases; Foodborne pathogens; Gastrointestinal tract; Gene expression; Health aspects; Infection; Infections; Intestine; Invasive species; Investigations; Poultry; Primary chicken intestinal cells; Probiotics; RNA
• ISSN: 1757-4749; 1757-4749
• DOI: 10.1186/s13099-024-00623-x

Campylobacter (C.) jejuni is one of the most important bacterial foodborne pathogens worldwide. Probiotics such as Lactobacillus or Bacillus species are considered one option for reducing the colonization rate and magnitude in poultry, the most frequent source of human infections. Due to the lack of suitable avian in vitro models such as chicken intestinal cell lines, especially those derived from the cecum, most in vitro studies on C. jejuni host interaction have been conducted with human intestinal cell lines. In this study, we compared C. jejuni-cell interactions between primary chicken cecal cells and the human intestinal cell line Caco-2, which is derived from colorectal adenocarcinoma, and investigated possible interfering effects of selected probiotic candidates. We detected differences in adhesion and invasion between the two tested gut cell types and between different C. jejuni strains. The probiotic inhibition of C. jejuni adhesion and invasion of human and avian gut cells was affected by host cell type, investigated C. jejuni strain and time points of probiotic treatment. Additionally, our results suggest a possible correlation between C. jejuni invasion and the detected increase in IL-6 mRNA expression. Our results indicate distinct differences between avian and human gut cells in their interaction with C. jejuni. Therefore, data obtained in one host species on C. jejuni-host interaction may not easily be transferrable to another one. The factors influencing the variable efficacy of probiotic intervention in chicken and human derived cells should be investigated further.