Effect of propolis gel on the in vitro reduction of dentin permeability

• Published in: Journal of applied oral science 2011-08, Vol.19 (4), p.318-323
• Main Authors: Sales-Peres, Silvia Helena de Carvalho, Carvalho, Flávia Negreiros de, Marsicano, Juliane Avansini, Mattos, Maria Cecília, Pereira, José Carlos, Forim, Moacir Rossi, Silva, Maria Fatima das Graças Fernandes da
• Format: Article
• Language: English
• Subjects: Analysis of Variance; Cariostatic Agents - chemistry; Cariostatic Agents - pharmacology; Dentin; Dentin - drug effects; Dentin - physiology; Dentin - ultrastructure; Dentin Desensitizing Agents - chemistry; Dentin permeability; Dentin Permeability - drug effects; Dentin sensitivity; Dentistry; DENTISTRY, ORAL SURGERY & MEDICINE; Fluorides - chemistry; Fluorides - pharmacology; Gels; Humans; In Vitro Techniques; Microscopy, Electron, Scanning; Original; Oxalates - chemistry; Oxalates - pharmacology; Propolis; Propolis - chemistry; Propolis - pharmacology; Smear Layer; Surface Properties; Water - chemistry
• ISSN: 1678-7757; 1678-7765; 1678-7765
• DOI: 10.1590/S1678-77572011005000004

The aim of this study was to evaluate the capacity of potassium oxalate, fluoride gel and two kinds of propolis gel to reduce the hydraulic conductance of dentin, in vitro. The methodology used for the measurement of hydraulic conductance of dentin in the present study was based on a model proposed in literature. Thirty-six 1-mm-thick dentin discs, obtained from extracted human third molars were divided into 4 groups (n=9). The groups corresponded to the following experimental materials: GI-10% propolis gel, pH 4.1; GII-30% propolis gel; GIII-3% potassium oxalate gel, pH 4,1; and GIV-1.23% fluoride gel, pH 4.1, applied to the dentin under the following surface conditions: after 37% phosphoric acid and before 6% citric acid application. The occluding capacity of the dentin tubules was evaluated using scanning electron microscopy (SEM) at ×500, ×1,000 and ×2,000 magnifications. Data were analyzed statistically by two-way ANOVA and Tukey's test at 5% significance level. Groups I, II, III, IV did not differ significantly from the others in any conditions by reducing in hydraulic conductance. The active agents reduced dentin permeability; however they produced the smallest reduction in hydraulic conductance when compared to the presence of smear layer (P