Scalable mapping of myelin and neuron density in the human brain with micrometer resolution

Optical coherence tomography (OCT) is an emerging 3D imaging technique that allows quantification of intrinsic optical properties such as scattering coefficient and back-scattering coefficient, and has proved useful in distinguishing delicate microstructures in the human brain. The origins of scatte...

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Bibliographic Details
Published in:Scientific reports 2022-01, Vol.12 (1), p.363-363, Article 363
Main Authors: Chang, Shuaibin, Varadarajan, Divya, Yang, Jiarui, Chen, Ichun Anderson, Kura, Sreekanth, Magnain, Caroline, Augustinack, Jean C., Fischl, Bruce, Greve, Douglas N., Boas, David A., Wang, Hui
Format: Article
Language:English
Subjects:
631/378/2606
631/378/87
Adult
Aged
Brain
Brain - cytology
Brain - diagnostic imaging
Brain - metabolism
Cadaver
Cell body
Female
Generalized linear models
Humanities and Social Sciences
Humans
Imaging, Three-Dimensional
Lasers
Male
Middle Aged
multidisciplinary
Myelin
Myelin Sheath
Neuroimaging
Neurons - chemistry
Optical properties
Predictive Value of Tests
Reproducibility of Results
Scattering coefficient
Scattering, Radiation
Science
Science (multidisciplinary)
Tomography, Optical Coherence
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Summary:Optical coherence tomography (OCT) is an emerging 3D imaging technique that allows quantification of intrinsic optical properties such as scattering coefficient and back-scattering coefficient, and has proved useful in distinguishing delicate microstructures in the human brain. The origins of scattering in brain tissues are contributed by the myelin content, neuron size and density primarily; however, no quantitative relationships between them have been reported, which hampers the use of OCT in fundamental studies of architectonic areas in the human brain and the pathological evaluations of diseases. Here, we built a generalized linear model based on Mie scattering theory that quantitatively links tissue scattering to myelin content and neuron density in the human brain. We report a strong linear relationship between scattering coefficient and the myelin content that is retained across different regions of the brain. Neuronal cell body turns out to be a secondary contribution to the overall scattering. The optical property of OCT provides a label-free solution for quantifying volumetric myelin content and neuron cells in the human brain.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-021-04093-y