Acetate Does Not Affect Palmitate Oxidation and AMPK Phosphorylation in Human Primary Skeletal Muscle Cells

Our recent human studies showed that colonic administration of sodium acetate (SA) resulted in increased circulating acetate levels, which was accompanied by increments in whole-body fat oxidation in overweight-obese men. Since skeletal muscle has a major role in whole-body fat oxidation, we aimed t...

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Bibliographic Details
Published in:Frontiers in endocrinology (Lausanne) 2021-06, Vol.12, p.659928-659928
Main Authors: González Hernández, Manuel A, Blaak, Ellen E, Hoebers, Nicole T H, Essers, Yvonne P G, Canfora, Emanuel E, Jocken, Johan W E
Format: Article
Language:English
Subjects:
acetate
Amino Acid Motifs
AMP-Activated Protein Kinases - chemistry
AMP-Activated Protein Kinases - metabolism
Cells, Cultured
Endocrinology
fat oxidation
gut metabolite
Humans
Insulin - metabolism
insulin sensitivity (IS)
Male
metabolic health
Middle Aged
Muscle Cells - drug effects
Muscle Cells - metabolism
Muscle, Skeletal - cytology
Muscle, Skeletal - drug effects
Muscle, Skeletal - metabolism
Oxidation-Reduction - drug effects
Palmitates - metabolism
Sodium Acetate - pharmacology
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Summary:Our recent human studies showed that colonic administration of sodium acetate (SA) resulted in increased circulating acetate levels, which was accompanied by increments in whole-body fat oxidation in overweight-obese men. Since skeletal muscle has a major role in whole-body fat oxidation, we aimed to investigate effects of SA on fat oxidation and underlying mechanisms in human primary skeletal muscle cells (HSkMC). We investigated the dose (0-5 mmol/L) and time (1, 4, 20, and 24 h) effect of SA on complete and incomplete endogenous and exogenous oxidation of C-labeled palmitate in HSkMC derived from a lean insulin sensitive male donor. Both physiological (0.1 and 0.25 mmol/L) and supraphysiological (0.5, 1 and 5 mmol/L) concentrations of SA neither increased endogenous nor exogenous fat oxidation over time in HSkMC. In addition, no effect of SA was observed on phosphorylation. In conclusion, our previously observed effects of SA on whole-body fat oxidation in men may not be explained direct effects on HSkMC fat oxidation. Nevertheless, SA-mediated effects on whole-body fat oxidation may be triggered by other mechanisms including gut-derived hormones or may occur in other metabolically active tissues.
ISSN:1664-2392
1664-2392
DOI:10.3389/fendo.2021.659928