Composition of Fecal Microbiota in Grazing and Feedlot Angus Beef Cattle

• Published in: Animals (Basel) 2021-11, Vol.11 (11), p.3167
• Main Authors: Zhang, Zhimin, Yang, Li, He, Yang, Luo, Xinmao, Zhao, Shaokang, Jia, Xianbo
• Format: Article
• Language: English
• Subjects: 16S rRNA gene; Abundance; Angus beef; Bacteria; Bacteroidetes; Beef; Beef cattle; Cattle; Composition; Consumers; Cyanobacteria; Diet; Elusimicrobia; Factory farming; fecal microbioa; Fecal microflora; Feces; Feedlots; Firmicutes; Genera; Grain; Grasslands; Grazing; grazing cattle; Meat; Meat quality; Microbiota; Next-generation sequencing; Relative abundance; rRNA 16S; Species diversity; Species richness; Taxonomy
• ISSN: 2076-2615; 2076-2615
• DOI: 10.3390/ani11113167

This study is to investigate the difference in bovine fecal microbiota between grazing and feedlot Angus cattle. Fecal samples were collected from six Angus cattle grazed on grassland and six Angus cattle fed on a feedlot. The fecal bacterial community was analyzed by high-throughput sequencing of 16S rRNA gene. Sequencing of the V3–V4 region totally produced 1,113,170 effective tages that were computationally clustered into 775 operational taxonomic units (OTUs). These 775 OTUs were taxonomically assigned to bacterial 12 phyla, 19 classes, 25 orders, 54 families, 141 genera, and 145 species. The dominant phyla were Firmicutes and Bacteroidetes. There was similar species richness between grazing and feedlot Angus beef, while higher species diversity was observed in feedlot Angus beef. The relative abundance of Firmicutes, Cyanobacteria, Elusimicrobia and Patescibacteria was significantly different between grazing and feedlot Angus beef (p < 0.05). At a genus level, five microbiotas were significantly different between the two groups and all belonged to the Firmicutes phylum. These significant differences in microbiota composition between grazing and feedlot Angus beef may have an impact on the meat quality of Angus beef.