New assessment of Anopheles vector species identification using MALDI-TOF MS

Anopheles species identification is essential for an effective malaria vector control programme. Matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry (MS) has been developed to identify adult Anopheles species, using the legs or the cephalothorax. The protein repe...

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Bibliographic Details
Published in:Malaria journal 2021-01, Vol.20 (1), p.33-33, Article 33
Main Authors: Nabet, Cécile, Kone, Abdoulaye K, Dia, Abdoulaye K, Sylla, Moussa, Gautier, Magali, Yattara, Mohammed, Thera, Mahamadou A, Faye, Ousmane, Braack, Leo, Manguin, Sylvie, Beavogui, Abdoul H, Doumbo, Ogobara, Gay, Frédérick, Piarroux, Renaud
Format: Article
Language:English
Subjects:
Acids
Anopheles
Anopheles gambiae
Aquatic insects
Cephalothorax
Evaluation
Head
Human diseases
Identification
Ionization
Lasers
Leg
Life Sciences
Malaria
Malaria vectors
MALDI-TOF MS
Mass spectrometry
Mass spectroscopy
Morphology
Mosquitoes
Properties
Proteins
Software
Species
Species identification
Standardization
Storage
Taxonomic identification
Taxonomy
Thorax
Tropical diseases
Vector-borne diseases
Wings
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Summary:Anopheles species identification is essential for an effective malaria vector control programme. Matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry (MS) has been developed to identify adult Anopheles species, using the legs or the cephalothorax. The protein repertoire from arthropods can vary according to compartment, but there is no general consensus regarding the anatomic part to be used. To determine the body part of the Anopheles mosquitoes best suited for the identification of field specimens, a mass spectral library was generated with head, thorax with wings and legs of Anopheles gambiae, Anopheles arabiensis and Anopheles funestus obtained from reference centres. The MSL was evaluated using two independent panels of 52 and 40 An. gambiae field-collected in Mali and Guinea, respectively. Geographic variability was also tested using the panel from Mali and several databases containing added specimens from Mali and Senegal. Using the head and a database without specimens from the same field collection, the proportion of interpretable and correct identifications was significantly higher than using the other body parts at a threshold value of 1.7 (p 
ISSN:1475-2875
1475-2875
DOI:10.1186/s12936-020-03557-2