The Epigenetic Modification of SLC5A8 in Papillary Thyroid Carcinoma and its Effects on Clinic-Pathological Features

Epigenetic alterations such as DNA methylation are known as the main cause of different types of cancers through inactivation of tumor suppressor genes, especially thyroid cancer. Identification of novel and effective markers are important in diagnosis and prevention of thyroid cancer. In the presen...

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Bibliographic Details
Published in:Iranian journal of public health 2022-03, Vol.51 (3), p.634-642
Main Authors: Sheikholeslami, Sara, Azizi, Fereidoun, Ghasemi, Asghar, Alibakhshi, Abbas, Parsa, Hossein, Shivaee, Setareh, Zarif-Yeganeh, Marjan, Hedayati, Mehdi, Teimoori-Toolabi, Ladan
Format: Article
Language:English
Subjects:
Cancer
CpG islands
Deoxyribonucleic acid
DNA
DNA methylation
Epigenetics
Gene expression
Genes
Goiter
Inactivation
mRNA
Mutation
Original
Papillary
Papillary thyroid carcinoma
Patients
Polymerase chain reaction
Thyroid
Thyroid cancer
Thyroidectomy
Tissues
Tumor suppressor
Tumor suppressor genes
Tumors
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Summary:Epigenetic alterations such as DNA methylation are known as the main cause of different types of cancers through inactivation of tumor suppressor genes, especially thyroid cancer. Identification of novel and effective markers are important in diagnosis and prevention of thyroid cancer. In the present study, the expression and methylation of Solute carrier family 5 member 8 ( ) in Papillary Thyroid Carcinoma (PTC) in comparison to multinodular goiter (MNG) have been studied. Overall, 41 patients with PTC and 36 patients affected by MNG were recruited from four hospitals in Tehran and Qazvin, Iran in 2018. Thyroid tissues were obtained during thyroidectomy. RNA and DNA were extracted from thyroid tissues. Quantitative RT-PCR assay was performed for determining the mRNA level of while Methylation-Sensitive High-Resolution Methylation was applied for assessing the methylation status. Methylation status of three regions composed of 52 CpG islands in the promoter of gene was studied by HRM assay. level in PTC tissues was significantly downregulated in average 0.4 fold in comparison with MNG tissues ( =0.05). The aberrant methylation of (b) region was remarkably different in PTC and MNG cases. The promoter methylation of (c) was significantly related to mutations and vascular invasion in PTC patients. The aberrant promoter hyper methylation of SLC5A8 was related to aggressive PTC. Therefore, there is some evidence to support the hypothesis that could be a paly important role in the development of PTC.
ISSN:2251-6085
2251-6093
DOI:10.18502/ijph.v51i3.8940