Molecular characteristics of optrA-carrying Enterococcus faecalis from chicken meat in South Korea

• Published in: Poultry science 2020-12, Vol.99 (12), p.6990-6996
• Main Authors: Yoon, Sunghyun, Son, Se Hyun, Kim, Yeong Bin, Seo, Kwang Won, Lee, Young Ju
• Format: Article
• Language: English
• Subjects: amino acids; aminoglycosides; Animals; Anti-Bacterial Agents - pharmacology; antibiotic resistance genes; antimicrobial resistance; chicken meat; Chickens - microbiology; Drug Resistance, Bacterial - genetics; Enterococcus faecalis; Enterococcus faecalis - drug effects; Enterococcus faecalis - genetics; fexA; Genes, Bacterial - genetics; linezolid; linezolid-resistance; macrolides; Meat - microbiology; Microbial Sensitivity Tests - veterinary; Microbiology and Food Safety; minimum inhibitory concentration; open reading frames; optrA; plasmids; Republic of Korea; sequence analysis; South Korea; tetracyclines; transposases; transposons
• ISSN: 0032-5791; 1525-3171
• DOI: 10.1016/j.psj.2020.08.062

The purpose of this study was to identify the genetic environment of optrA gene in linezolid (LZD)-resistant Enterococcus faecalis from chicken meat and to describe the probable mechanism of dissemination of the optrA gene through plasmid or chromosomal integration. Whole genome sequencing and analysis revealed that all 3 E. faecalis isolates confirmed as LZD- and chloramphenicol-resistant carried fexA adjacent to the optrA gene as well as a variety of resistance genes for macrolides, tetracyclines, and aminoglycosides, simultaneously. But, the other genes conferring LZD resistance, cfr and poxtA, were not detected in those strains. Two isolates harboring the optrA gene in their chromosomal DNA showed >99% similarity in arrangement to the transposon Tn6674 and the transposase genes, tnpA, tnpB, and tnpC and were located in the first open reading frame for transposase. One isolate harboring an optrA-carrying plasmid also showed >99% similarity with the previously reported pE439 plasmid but had 2 amino acid changes (Thr96Lys and Tyr160Asp) and a higher minimum inhibitory concentration against LZD of 16 mg/L than that of pE439 (8 mg/L). Mobile genetic elements such as transposons or plasmids flanking the optrA gene conduct a crucial role in the dissemination of antimicrobial resistance genes. Further investigations are required to identify the way by which optrA is integrated into chromosomal DNA and plasmids.