Non-invasive monitoring of T cell differentiation through Raman spectroscopy

The monitoring of dynamic cellular behaviors remains a technical challenge for most established techniques used nowadays for single-cell analysis, as most of them are either destructive, or rely on labels that can affect the long-term functions of cells. We employ here label-free optical techniques...

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Bibliographic Details
Published in:Scientific reports 2023-02, Vol.13 (1), p.3129-3129, Article 3129
Main Authors: Pavillon, Nicolas, Smith, Nicholas I.
Format: Article
Language:English
Subjects:
631/250/1619/554/1834
631/250/1619/554/1898
639/624/1107/328
639/624/1107/527/1821
Animals
Cell activation
Cell Differentiation
Dyes
Effector cells
Humanities and Social Sciences
Lymphocytes
Lymphocytes T
Mathematical models
Measurement techniques
Methods
Mice
multidisciplinary
Raman spectroscopy
Science
Science (multidisciplinary)
Spectrum analysis
Spectrum Analysis, Raman - methods
Statistical analysis
Statistical models
Surface markers
T cell receptors
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Summary:The monitoring of dynamic cellular behaviors remains a technical challenge for most established techniques used nowadays for single-cell analysis, as most of them are either destructive, or rely on labels that can affect the long-term functions of cells. We employ here label-free optical techniques to non-invasively monitor the changes that occur in murine naive T cells upon activation and subsequent differentiation into effector cells. Based on spontaneous Raman single-cell spectra, we develop statistical models that allow the detection of activation, and employ non-linear projection methods to delineate the changes occurring over a several day period spanning early differentiation. We show that these label-free results have very high correlation with known surface markers of activation and differentiation, while also providing spectral models that allow the identification of the underlying molecular species that are representative of the biological process under study.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-023-29259-8