A LAT1-Like Amino Acid Transporter Regulates Neuronal Activity in the Drosophila Mushroom Bodies

The proper functioning of neural circuits that integrate sensory signals is essential for individual adaptation to an ever-changing environment. Many molecules can modulate neuronal activity, including neurotransmitters, receptors, and even amino acids. Here, we ask whether amino acid transporters e...

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Bibliographic Details
Published in:Cells (Basel, Switzerland) Switzerland), 2024-08, Vol.13 (16), p.1340
Main Authors: Delescluse, Julie, Simonnet, Mégane M, Ziegler, Anna B, Piffaretti, Kévin, Alves, Georges, Grosjean, Yael, Manière, Gérard
Format: Article
Language:English
Subjects:
Amino Acid Transport Systems - genetics
Amino Acid Transport Systems - metabolism
Amino acids
Amino Acids - metabolism
Animals
Brain
Calcium imaging
Diet
Drosophila
Drosophila melanogaster - metabolism
Drosophila Proteins - genetics
Drosophila Proteins - metabolism
Ethylenediaminetetraacetic acid
Glial cells
Glutamate
Glutamate dehydrogenase
Insects
Kinases
LAT-1
Life Sciences
Metabolism
Mushroom bodies
Mushroom Bodies - metabolism
Neural networks
Neurobiology
Neuroimaging
Neuronal-glial interactions
Neurons
Neurons - metabolism
Neurons and Cognition
Neurophysiology
Rapamycin
Signal Transduction
SLC
solute carrier
TOR protein
TOR Serine-Threonine Kinases - metabolism
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Summary:The proper functioning of neural circuits that integrate sensory signals is essential for individual adaptation to an ever-changing environment. Many molecules can modulate neuronal activity, including neurotransmitters, receptors, and even amino acids. Here, we ask whether amino acid transporters expressed by neurons can influence neuronal activity. We found that ( ), which encodes a light chain of a heterodimeric amino acid transporter, is expressed in different cell types of the adult brain: in mushroom body neurons (MBs) and in glial cells. Using live calcium imaging, we found that MND expressed in α/β MB neurons is essential for sensitivity to the L-amino acids: Leu, Ile, Asp, Glu, Lys, Thr, and Arg. We found that the Target Of Rapamycin (TOR) pathway but not the Glutamate Dehydrogenase (GDH) pathway is involved in the Leucine-dependent response of α/β MB neurons. This study strongly supports the key role of MND in regulating MB activity in response to amino acids.
ISSN:2073-4409
2073-4409
DOI:10.3390/cells13161340