Oil Red O Staining for Lipid Content in Caenorhabditis elegans

The nematode has emerged as a popular model system for studying the regulation of lipid metabolism. Therefore, it is critical to develop a method for determining fat storage in individual worms. Oil Red O (ORO) staining has been validated as an accurate assessment for major fat storage in . Here, we...

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Bibliographic Details
Published in:Bio-protocol 2021-08, Vol.11 (16), p.e4124-e4124
Main Authors: Wang, Feng-Yung, Ching, Tsui-Ting
Format: Article
Language:English
Subjects:
Biology
Methods
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Summary:The nematode has emerged as a popular model system for studying the regulation of lipid metabolism. Therefore, it is critical to develop a method for determining fat storage in individual worms. Oil Red O (ORO) staining has been validated as an accurate assessment for major fat storage in . Here, we describe an optimized protocol for ORO staining of and provide detailed instructions for quantifying the intensity of ORO signal in images acquired by light microscopy.
ISSN:2331-8325
2331-8325
DOI:10.21769/BioProtoc.4124