Protective mechanism of turmeric (Curcuma longa) on carbofuran-induced hematological and hepatic toxicities in a rat model

• Published in: Pharmaceutical biology 2017, Vol.55 (1), p.1937-1945
• Main Authors: Hossen, Md. Sakib, Tanvir, E. M., Prince, Maruf Billah, Paul, Sudip, Saha, Moumoni, Ali, Md. Yousuf, Gan, Siew Hua, Khalil, Md. Ibrahim, Karim, Nurul
• Format: Article
• Language: English
• Subjects: Animal models; Animals; Antioxidant; antioxidant activity; antioxidant enzymes; Antioxidants; Antioxidants - isolation & purification; Antioxidants - pharmacology; Ascorbic acid; Biological activity; Blood Cells - drug effects; Blood Cells - metabolism; Blood Cells - pathology; Carbofuran; Carbofuran - toxicity; Curcuma; Curcuma longa; Dietary supplements; Dose-Response Relationship, Drug; DPPH; Enzymes; Erythrocytes - drug effects; Erythrocytes - metabolism; Erythrocytes - pathology; Flavonoids; FRAP; Free radicals; Glutathione peroxidase; Hematology; Hemoglobin; hepatic markers; histopathology; Iron; leukocytes; Leukocytes - drug effects; Leukocytes - metabolism; Leukocytes - pathology; Lipid peroxidation; Liver; Liver - drug effects; Liver - metabolism; Liver - pathology; Male; males; Models, Animal; Oral administration; Original; Oxidative stress; Oxidative Stress - drug effects; Oxidative Stress - physiology; pesticide; Plant Extracts - isolation & purification; Plant Extracts - pharmacology; Polyphenols; protective effect; Random Allocation; Rats; Rats, Wistar; Rodents; Superoxide dismutase; Toxicity; turmeric
• ISSN: 1388-0209; 1744-5116; 1744-5116
• DOI: 10.1080/13880209.2017.1345951

Context: Turmeric (Curcuma longa L. [Zingiberaceae]) is used in the treatment of a variety of conditions including pesticide-induced toxicity. Objective: The study reports the antioxidant properties and the protective effects of turmeric against carbofuran (CF)-induced toxicity in rats. Materials and methods: The antioxidant potential was determined by using free radicals scavenging activity and ferric reducing antioxidant power values. Male Wistar rats were randomly divided into four groups, designated as control, turmeric (100 mg/kg/day), CF (1 mg/kg/day) and turmeric (100 mg/kg/day) + CF (1 mg/kg/day) treatments. All of the doses were administered orally for 28 consecutive days. The biological activity of the turmeric and CF was determined by using several standard biochemical methods. Results: Turmeric contains high concentrations of polyphenols (8.97 ± 0.15 g GAEs), flavonoids (5.46 ± 0.29 g CEs), ascorbic acid (0.06 ± 0.00 mg AEs) and FRAP value (1972.66 ± 104.78 μM Fe 2+ ) per 100 g of sample. Oral administration of CF caused significant changes in some of the blood indices, such as, mean corpuscular volume, corpuscular hemoglobin, white blood cell, platelet distribution width and induced severe hepatic injuries associated with oxidative stress, as observed by the significantly higher lipid peroxidation (LPO) levels when compared to control, while the activities of cellular antioxidant enzymes (including superoxide dismutase and glutathione peroxidase) were significantly suppressed in the liver tissue. Discussion and conclusion: Turmeric supplementation could protect against CF-induced hematological perturbations and hepatic injuries in rats, plausibly by the up-regulation of antioxidant enzymes and inhibition of LPO to confer the protective effect.