Loading…

Toxicological effects and resistance to pyrethroids in Boophilus microplus from Goiás, Brazil Efeitos toxicológicos e resistência a piretróides em Boophilus microplus de Goiás

In order to monitor the susceptibility of Boophilus microplus to acaricides and improve control measures, the effects of cypermethrin, deltamethrin and permethrin on larvae obtained in the city of Goiânia in the Brazilian state of Goiás were studied. Although these pyrethroids are already sold as ac...

Full description

Saved in:
Bibliographic Details
Published in:Arquivo brasileiro de medicina veterinária e zootecnia 2001-10, Vol.53 (5), p.538-543
Main Author: F.F. Fernandes
Format: Article
Language:English
Subjects:
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:In order to monitor the susceptibility of Boophilus microplus to acaricides and improve control measures, the effects of cypermethrin, deltamethrin and permethrin on larvae obtained in the city of Goiânia in the Brazilian state of Goiás were studied. Although these pyrethroids are already sold as acaricides, their cost-benefit efficiency has been questioned. Fasting 14-21 day-old larvae were immersed in solutions of the acaricides under test, maintained at 27±1° C, and relative humidity over 80%, and observed under the stereoscope within an apparatus originally designed for studying the non-parasitic phase of the tick life cycle. The observed toxicological effects were: excitability, repetitive motion, decreased motor ability, detachment, paralysis, knock-down and cuticular proliferation of liquids and gases. The materials used in the manufacture of this apparatus consisted of disposable Petri dishes, "organza" cloth and paraffin, none of which are toxic to tick larvae. Mean death rates after 24h were 76.3%, 87.5%, 77.6%, 91.2%, 86.2% and 100% for 25 and 50ppm deltamethrin, 150 and 300ppm cypermethrin and 1250 and 2500ppm permethrin, respectively. The ticks were resistant to commercial concentrations of deltamethrin and cypermethrin. Only 2500ppm permethrin produced the mortality recommended by the Brazilian Ministry of Agriculture.Estudaram-se os efeitos de cipermetrina, deltametrina e permetrina sobre larvas de uma cepa de campo de Goiânia, com o objetivo de monitorar a susceptibilidade de Boophilus microplus para esses acaricidas e fomentar medidas de controle. Larvas em jejum com 14 a 21 dias, imersas em soluções desses piretróides, foram mantidas a 27±1°C e UR% > ou = 80% e observadas por 24h ao estereoscópio, contidas em dispositivo desenvolvido originalmente para estudos da fase não parasitária do ciclo evolutivo. O material utilizado em sua confecção, placa de petri descartável, tecido organza e parafina, não foi tóxico para as larvas. Os seguintes efeitos toxicológicos foram observados: excitabilidade, movimentação repetitiva, diminuição da capacidade locomotora, desprendimento, paralisia, knock-down e proliferação cuticular de líquidos e gases. A mortalidade média na 24ªh foi de 76,3%, 87,5%, 77,6%, 91,2%, 86,20%, e 100,0%, respectivamente, para deltametrina 25ppm e 50ppm, cipermetrina 150ppm e 300ppm e permetrina 1250ppm e 2500ppm. O ixodídeo foi resistente às concentrações comerciais de deltametrina e cipermetrina. Apenas permetrina 2500ppm pro
ISSN:0102-0935
1678-4162
DOI:10.1590/S0102-09352001000500004