Biocontrol of rusted root rot in Panax ginseng by a combination of extracts from Bacillus amyloliquefaciens YY8 crude protein and Enterobacteriaceae YY115 ethyl acetate

• Published in: BMC microbiology 2024-09, Vol.24 (1), p.317-15, Article 317
• Main Authors: Feng, Shuaiqi, Zhao, Yuchi, Wang, Qiuyu, Zhang, Jiyue, Liang, Xue, Fu, Zhuoyue, Li, Yanjie, Dong, Weiwei, Ji, Wenxiu
• Format: Article
• Language: English
• Subjects: Acetates; Acetates - pharmacology; Acetic acid; Agricultural development; Antifungal activity; Antifungal agents; Antifungal Agents - metabolism; Antifungal Agents - pharmacology; Ascomycota - chemistry; Ascomycota - drug effects; Bacillus amyloliquefaciens; Bacillus amyloliquefaciens - chemistry; Bacillus amyloliquefaciens - metabolism; Bacillus amyloliquefaciens - physiology; Bacteria; Bacterial Proteins - genetics; Bacterial Proteins - metabolism; Biological control; Biological Control Agents - pharmacology; Biological effects; Care and treatment; Cell membranes; Chemical pest control; Cylindrocarpon destructans; Diagnosis; Diseases; Electric properties; Electrical conductivity; Electrical resistivity; Electrolytes; Electrolytic cells; Enterobacteriaceae - drug effects; Esters; Ethyl acetate; Fermentation; Flowers & plants; Fungal diseases of plants; Fungi; Fungicides; Genetic aspects; Ginseng; Growth; High temperature; Membrane disruption; Microorganisms; Morphology; Nucleic acids; Panax - chemistry; Panax - microbiology; Panax ginseng; Pathogens; Pesticides; Pests; Plant diseases; Plant Diseases - microbiology; Plant Diseases - prevention & control; Plant Roots - microbiology; Proteins; Ratios; Rhizosphere; Root rot; Rusted root rot; Soil bacteria; Soil Microbiology; Soil microorganisms; Strains (organisms); Sustainable development; Toxicity; Yeast
• ISSN: 1471-2180; 1471-2180
• DOI: 10.1186/s12866-024-03475-2

Rusted root rot is one of the most common root diseases in Panax ginseng, and Cylindrocarpon destructans is one of the main pathogenic fungus. The objective of this study was to screen and explore the extracts of biocontrol bacteria isolated from ginseng rhizosphere soil against Cylindrocarpon destructans. Bacterial strains Bacillus amyloliquefaciens YY8 and Enterobacteriacea YY115 were isolated and found to exhibit in vitro antifungal activity against C. destructans. A combination of crude protein extract from B. amyloliquefaciens YY8 and ethyl acetate extract from Enterobacteriacea YY115 in a 6:4 ratio exhibited the strongest antifungal activity against C. destructans. Measurements of electrical conductivity, protein content, and nucleic acid content in suspension cultures of C. destructans treated with a mixture extracts indicated that the extracts disrupted the cell membranes of rusted root rot mycelia, resulting in the leakage of electrolytes, proteins, and nucleic acids from the cells, and ultimately inhibiting the growth of C. destructans. The combined extracts suppressed the infection of ginseng roots discs by C. destructans effectively. The extracts obtained from the two bacterial strains effectively inhibited C. destructans in P. ginseng. It can provide scientific basis for the development of new biological control pesticides, reduce the use of chemical pesticides, and promote the sustainable development of agriculture.