Preparation and characterization of solid lipid nanoparticles loaded with frankincense and myrrh oil

• Published in: International journal of nanomedicine 2012-01, Vol.7 (default), p.2033-2043
• Main Authors: Shi, Feng, Zhao, Ji-Hui, Liu, Ying, Wang, Zhi, Zhang, Yong-Tai, Feng, Nian-Ping
• Format: Article
• Language: English
• Subjects: Administration, Oral; Animals; Antineoplastic Agents, Phytogenic - administration & dosage; Antineoplastic Agents, Phytogenic - chemistry; antitumor activity; Boswellia - chemistry; Chemical properties; Chemistry, Pharmaceutical; Drug Carriers - chemistry; Drug delivery systems; Drugs; Drugs, Chinese Herbal - administration & dosage; Drugs, Chinese Herbal - chemistry; evaporation release; frankincense oil; Hydrophobic and Hydrophilic Interactions; Lipids; Lipids - chemistry; Male; Mice; myrrh oil; Nanomedicine; Nanoparticles; Nanoparticles - administration & dosage; Nanoparticles - chemistry; Nanoparticles - ultrastructure; Neoplasms, Experimental - drug therapy; Neoplasms, Experimental - pathology; Oils, Volatile - administration & dosage; Oils, Volatile - chemistry; Original Research; Particle Size; Plant Oils - administration & dosage; Plant Oils - chemistry; Properties; solid lipid nanoparticles; Terpenes - administration & dosage; Thermodynamics; traditional Chinese medicine; Vehicles
• ISSN: 1178-2013; 1176-9114; 1178-2013
• DOI: 10.2147/IJN.S30085

The aim of the present study was to prepare solid lipid nanoparticles (SLNs) for the oral delivery of frankincense and myrrh essential oils (FMO). Aqueous dispersions of SLNs were successfully prepared by a high-pressure homogenization method using Compritol 888 ATO as the solid lipid and soybean lecithin and Tween 80 as the surfactants. The properties of the SLNs such as particle size, zeta potential (ZP), and drug encapsulation efficiency (EE) were investigated. The morphology of SLNs was observed by transmission electron microscopy (TEM). The crystallinity of the formulation was analyzed by differential scanning calorimetry (DSC) and X-ray diffraction (XRD). In addition, drug evaporation release and antitumor activity were also studied. Round SLNs with a mean size of 113.3 ± 3.6 nm, a ZP of -16.8 ± 0.4 mV, and an EE of 80.60% ± 1.11% were obtained. DSC and XRD measurements revealed that less ordered structures were formed in the inner cores of the SLN particles. Evaporation loss of the active components in FMO could be reduced in the SLNs. Furthermore, the SLN formulation increased the antitumor efficacy of FMO in H22-bearing Kunming mice. Hence, the presented SLNs can be used as drug carriers for hydrophobic oil drugs extracted from traditional Chinese medicines.