Production of Bioactive Compounds with Broad Spectrum Bactericidal Action, Bio-Film Inhibition and Antilarval Potential by the Secondary Metabolites of the Endophytic Fungus Cochliobolus sp. APS1 Isolated from the Indian Medicinal Herb Andrographis paniculata

• Published in: Molecules (Basel, Switzerland) Switzerland), 2022-02, Vol.27 (5), p.1459
• Main Authors: Santra, Hiran Kanti, Maity, Santanu, Banerjee, Debdulal
• Format: Article
• Language: English
• Subjects: 1-(2-aminoethyl); Acetic acid; Alkaloids; Andrographis - chemistry; Andrographis paniculata; Animals; Anti-Bacterial Agents - biosynthesis; Anti-Bacterial Agents - chemistry; Anti-Bacterial Agents - pharmacology; anti-biofilm; antibacterial; Antibacterial activity; Antibiotics; Antimicrobial agents; Ascomycota - metabolism; aziridine; Bioactive compounds; Biofilms; Biofilms - drug effects; Biofilms - growth & development; Biological activity; Cochliobolus; Coevolution; Drug resistance; Endophytes; Endophytes - chemistry; Endophytes - metabolism; Enzymes; Ethyl acetate; Flowers & plants; Fungi; Herbal medicine; Host plants; Larva - microbiology; larvicidal potency; Macromolecules; Medical equipment; Metabolites; Microbial Sensitivity Tests; Microorganisms; Minimum inhibitory concentration; Natural products; optimization; Pathogens; Pharmaceuticals; Plants, Medicinal - microbiology; Potatoes; Response surface methodology; Secondary metabolites; Staphylococcus infections; Urea; Vector-borne diseases
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules27051459

Endophytes, being the co-evolution partners of green host plants, are factories of pharmaceutically valuable novel natural products. sp. APS1, an endophyte of (Green Chiretta), produces a plethora of natural bioactive compounds and the multipotent alkaloid Aziridine, 1-(2-aminoethyl)-, is the prime one among them. The isolate exhibited antibacterial, anti-biofilm, and antilarval potency. The MIC and MBC values of the ethyl-acetate culture extract ranged from 15.62 to 250 µg/mL against ten pathogenic microorganisms (including MRSA and VRSA). Killing kinetics data along with the leakage of macromolecules into the extracellular environment supports the cidal activity of the antibacterial principles. The broad spectrum antibacterial activity of Aziridine, 1-(2-aminoethyl)-, was optimized by a one-variable-at-a-time system coupled with response surface methodology, which led to a 45% enhancement of the antibacterial activity. The maximum response (22.81 ± 0.16 mm of zone of inhibition against MRSA) was marked in 250 mL Erlenmeyer flask containing 90 mL potato dextrose broth supplemented with (g%/L) glucose, 9.7; urea concentration, 0.74; with medium pH 6.48; after 8.76 days of incubation at 26 °C. APS1 strongly inhibited biofilm formation in the tested pathogenic microorganisms and acts as a larvicidal agent against the Dengue-vector . This is probably the first report of Aziridine, 1-(2-aminoethyl)-, from any endophytic source. sp. APS1 possesses industrial importance for the production of bioactive alkaloids.