Quantification and Optimization of Ethanolic Extract Containing the Bioactive Flavonoids from Millettia pulchra Radix

Millettia pulchra is traditionally used for treating diseases, including joint pain, fever, anemia, and allergies. It is also a potential resource of natural flavonoid derivatives, which represents major constituents of this plant. This study aimed to isolate the major compounds from M. pulchra radi...

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Bibliographic Details
Published in:Molecules (Basel, Switzerland) Switzerland), 2021-06, Vol.26 (12), p.3641
Main Authors: Vo, Thanh-Hoa, Liaw, Chia-Ching, Lin, Yu-Chi, Nguyen, Duc Hanh, Nguyen, Thi Tuyet Nhung, Lee, Ching-Kuo, Kuo, Yao-Haur
Format: Article
Language:English
Subjects:
Allergies
Anemia
artificial neural network
Cancer
Cell cycle
Column chromatography
Crystallization
Ethanol
Fever
Flavonoids
High performance liquid chromatography
Kinases
Liquid chromatography
Neural networks
Optimization
Pain
Photodiodes
Quality control
quantification
Response surface methodology
Silica
Silica gel
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Summary:Millettia pulchra is traditionally used for treating diseases, including joint pain, fever, anemia, and allergies. It is also a potential resource of natural flavonoid derivatives, which represents major constituents of this plant. This study aimed to isolate the major compounds from M. pulchra radix, develop and validate the HPLC-PDA method to determine their contents, and optimize its extraction. Four major flavonoid derivatives (karanjin, lanceolatin B, 2”,2”-dimethylpyrano-[5″,6″:7,8]-flavone, and pongamol) were isolated using silica gel column chromatography, crystallization techniques in large amounts with high purities (>95%). A simple, accurate high-performance liquid chromatography–photodiode array (HPLC–PDA) detection method has been developed and validated with significantly statistical impacts according to International Conference on Harmonization (ICH) guidelines. The Response Surface Methodology (RSM), Artificial Neural Network (ANN) models were employed to predictive performance and optimization of the extraction process. The optimized conditions for the extraction of major flavonoids were: extraction time (twice), solvent/material ratio (9.5), and ethanol concentration (72.5%). Our research suggests an effective method, which will be helpful for quality control in the pharmaceutical development of this species.
ISSN:1420-3049
1420-3049
DOI:10.3390/molecules26123641