A Nuclear Magnetic Resonance-Based Metabolomic Study to Identify Metabolite Differences between Iranian Isolates of Leishmania major and Leishmania tropica

Cutaneous leishmaniasis caused by species ( ) is one of the most important parasitic diseases in humans. To gain information on the metabolite variations and biochemical pathways between , we used the comparative metabolome of metacyclic promastigotes in the Iranian isolates of and by proton nuclear...

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Bibliographic Details
Published in:Iranian journal of medical sciences 2021-01, Vol.46 (1), p.43-51
Main Authors: Tabrizi, Fatemeh, Seyyed Tabaei, Seyyed Javad, Ali Ahmadi, Nayeb, Arefi Oskouie, Afsaneh
Format: Article
Language:English
Subjects:
Aspartate
Comparative analysis
Discriminant analysis
leishmania major
leishmania tropica
Leishmaniasis
Metabolites
metabolomics
NMR
Nuclear magnetic resonance
Nuclear magnetic resonance spectroscopy
Original
Parasitic diseases
proton magnetic resonance spectroscopy
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Summary:Cutaneous leishmaniasis caused by species ( ) is one of the most important parasitic diseases in humans. To gain information on the metabolite variations and biochemical pathways between , we used the comparative metabolome of metacyclic promastigotes in the Iranian isolates of and by proton nuclear magnetic resonance ( H-NMR). and were collected from three areas of Iran, namely Gonbad, Mashhad, and Bam, between 2017 and 2018, and were cultured. The metacyclic promastigote of each species was separated, and cell metabolites were extracted. H-NMR spectroscopy was applied, and the data were processed using ProMatab in MATLAB (version 7.8.0.347). Multivariate statistical analyses, including the principal component analysis and the orthogonal projections to latent structures discriminant analysis, were performed to identify the discriminative metabolites between the two . Metabolites with variable influences in projection values of more than one and a P value of less than 0.05 were marked as significant differences. A set of metabolites were detected, and 24 significantly differentially expressed metabolites were found between the metacyclic forms of and isolates. The top differential metabolites were methionine, aspartate, betaine, and acetylcarnitine, which were increased more in than (P
ISSN:0253-0716
1735-3688
DOI:10.30476/ijms.2019.82120.0