Immune response of DNA vaccinated-gilthead seabream ( Sparus aurata ) against LCDV-Sa infection: relevance of the inflammatory process

Lymphocystis disease is one of the main viral pathologies affecting cultured gilthead seabream ( ) in the Mediterranean region. Recently, we have developed a DNA vaccine based on the major capsid protein (MCP) of the (LCDV-Sa). The immune response triggered by either LCDV-Sa infection or vaccination...

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Bibliographic Details
Published in:Frontiers in immunology 2023-06, Vol.14, p.1209926-1209926
Main Authors: Leiva-Rebollo, Rocio, Gémez-Mata, Juan, Castro, Dolores, Borrego, Juan J, Labella, Alejandro M
Format: Article
Language:English
Subjects:
Animals
DNA
DNA Virus Infections
DNA-vaccine
gilthead seabream
Immunity
Immunology
inflammation
innate immune response
Iridoviridae - physiology
LCDV-Sa
OpenArray
Sea Bream
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Summary:Lymphocystis disease is one of the main viral pathologies affecting cultured gilthead seabream ( ) in the Mediterranean region. Recently, we have developed a DNA vaccine based on the major capsid protein (MCP) of the (LCDV-Sa). The immune response triggered by either LCDV-Sa infection or vaccination have been previously studied and seem to be highly related to the modulation of the inflammatory and the IFN response. However, a comprehensive evaluation of immune-related gene expression in vaccinated fish after viral infection to identify immunogenes involved in vaccine-induced protection have not been carried out to date. The present study aimed to fulfill this objective by analyzing samples of head-kidney, spleen, intestine, and caudal fin from fish using an OpenArray platform containing targets related to the immune response of gilthead seabream. The results obtained showed an increase of deregulated genes in the hematopoietic organs between vaccinated and non-vaccinated fish. However, in the intestine and fin, the results showed the opposite trend. The global effect of fish vaccination was a significant decrease (
ISSN:1664-3224
1664-3224
DOI:10.3389/fimmu.2023.1209926