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Immune response of DNA vaccinated-gilthead seabream ( Sparus aurata ) against LCDV-Sa infection: relevance of the inflammatory process
Lymphocystis disease is one of the main viral pathologies affecting cultured gilthead seabream ( ) in the Mediterranean region. Recently, we have developed a DNA vaccine based on the major capsid protein (MCP) of the (LCDV-Sa). The immune response triggered by either LCDV-Sa infection or vaccination...
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| Published in: | Frontiers in immunology 2023-06, Vol.14, p.1209926-1209926 |
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| creator | Leiva-Rebollo, Rocio Gémez-Mata, Juan Castro, Dolores Borrego, Juan J Labella, Alejandro M |
| description | Lymphocystis disease is one of the main viral pathologies affecting cultured gilthead seabream (
) in the Mediterranean region. Recently, we have developed a DNA vaccine based on the major capsid protein (MCP) of the
(LCDV-Sa). The immune response triggered by either LCDV-Sa infection or vaccination have been previously studied and seem to be highly related to the modulation of the inflammatory and the IFN response. However, a comprehensive evaluation of immune-related gene expression in vaccinated fish after viral infection to identify immunogenes involved in vaccine-induced protection have not been carried out to date. The present study aimed to fulfill this objective by analyzing samples of head-kidney, spleen, intestine, and caudal fin from fish using an OpenArray
platform containing targets related to the immune response of gilthead seabream. The results obtained showed an increase of deregulated genes in the hematopoietic organs between vaccinated and non-vaccinated fish. However, in the intestine and fin, the results showed the opposite trend. The global effect of fish vaccination was a significant decrease ( |
| doi_str_mv | 10.3389/fimmu.2023.1209926 |
| format | article |
| fullrecord | <record><control><sourceid>proquest_doaj_</sourceid><recordid>TN_cdi_doaj_primary_oai_doaj_org_article_20e56f19ac0049a0ae68265cb6e931a5</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><doaj_id>oai_doaj_org_article_20e56f19ac0049a0ae68265cb6e931a5</doaj_id><sourcerecordid>2828759029</sourcerecordid><originalsourceid>FETCH-LOGICAL-c469t-eee66b187fed8cdb26351a98cbb8abe698eda4b5d68e6ab9c81598562a68af4f3</originalsourceid><addsrcrecordid>eNpVkk1v1DAQhiMEolXpH-CAfCyHLLEde20uqNrysdIKDgWu1tiZbF0lcbCdlfoH-N1kP6jauXjkmXnmlf0WxVtaLThX-kPr-35asIrxBWWV1ky-KM6plHXJGatfPsnPisuU7qs5as05F6-LM77ktaxqcV78Xc-YAUnENIYhIQktufl-TXbgnB8gY1NufZfvEBqSEGxE6MkVuR0hTonAFCEDeU9gC35ImWxWN7_LWyB-aNFlH4aPM7nDHQzugJ5B-1oHfQ85xAcyxuAwpTfFqxa6hJen86L49eXzz9W3cvPj63p1vSldLXUuEVFKS9WyxUa5xjLJBQWtnLUKLEqtsIHaikYqlGC1U1RoJSQDqaCtW35RrI_cJsC9GaPvIT6YAN4cLkLcGojZuw4Nq1DIlmpw-4eDClAqJoWzEjWnIGbWpyNrnGyPjcMhR-ieQZ9XBn9ntmFnaMWWs6p6JlydCDH8mTBl0_vksOtgwDAlwxRTS6ErpudWdmx1MaQUsX3cQyuzN4Q5GMLsDWFOhpiH3j1V-Djy__v5P7TktPM</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2828759029</pqid></control><display><type>article</type><title>Immune response of DNA vaccinated-gilthead seabream ( Sparus aurata ) against LCDV-Sa infection: relevance of the inflammatory process</title><source>PubMed Central</source><creator>Leiva-Rebollo, Rocio ; Gémez-Mata, Juan ; Castro, Dolores ; Borrego, Juan J ; Labella, Alejandro M</creator><creatorcontrib>Leiva-Rebollo, Rocio ; Gémez-Mata, Juan ; Castro, Dolores ; Borrego, Juan J ; Labella, Alejandro M</creatorcontrib><description>Lymphocystis disease is one of the main viral pathologies affecting cultured gilthead seabream (
) in the Mediterranean region. Recently, we have developed a DNA vaccine based on the major capsid protein (MCP) of the
(LCDV-Sa). The immune response triggered by either LCDV-Sa infection or vaccination have been previously studied and seem to be highly related to the modulation of the inflammatory and the IFN response. However, a comprehensive evaluation of immune-related gene expression in vaccinated fish after viral infection to identify immunogenes involved in vaccine-induced protection have not been carried out to date. The present study aimed to fulfill this objective by analyzing samples of head-kidney, spleen, intestine, and caudal fin from fish using an OpenArray
platform containing targets related to the immune response of gilthead seabream. The results obtained showed an increase of deregulated genes in the hematopoietic organs between vaccinated and non-vaccinated fish. However, in the intestine and fin, the results showed the opposite trend. The global effect of fish vaccination was a significant decrease (
<0.05) of viral replication in groups of fish previously vaccinated, and the expression of the following immune genes related to viral recognition (
), humoral and cellular response (
and
), inflammation (
,
,
, and
), antiviral response (
,
,
,
), cell-mediated cytotoxicity (
), and apoptosis (
). The exclusive modulation of the immune response provoked by the vaccination seems to control the progression of the infection in the experimentally challenged gilthead seabream.</description><identifier>ISSN: 1664-3224</identifier><identifier>EISSN: 1664-3224</identifier><identifier>DOI: 10.3389/fimmu.2023.1209926</identifier><identifier>PMID: 37346045</identifier><language>eng</language><publisher>Switzerland: Frontiers Media S.A</publisher><subject>Animals ; DNA ; DNA Virus Infections ; DNA-vaccine ; gilthead seabream ; Immunity ; Immunology ; inflammation ; innate immune response ; Iridoviridae - physiology ; LCDV-Sa ; OpenArray ; Sea Bream</subject><ispartof>Frontiers in immunology, 2023-06, Vol.14, p.1209926-1209926</ispartof><rights>Copyright © 2023 Leiva-Rebollo, Gémez-Mata, Castro, Borrego and Labella.</rights><rights>Copyright © 2023 Leiva-Rebollo, Gémez-Mata, Castro, Borrego and Labella 2023 Leiva-Rebollo, Gémez-Mata, Castro, Borrego and Labella</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c469t-eee66b187fed8cdb26351a98cbb8abe698eda4b5d68e6ab9c81598562a68af4f3</citedby><cites>FETCH-LOGICAL-c469t-eee66b187fed8cdb26351a98cbb8abe698eda4b5d68e6ab9c81598562a68af4f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC10279854/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC10279854/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,27922,27923,53789,53791</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/37346045$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Leiva-Rebollo, Rocio</creatorcontrib><creatorcontrib>Gémez-Mata, Juan</creatorcontrib><creatorcontrib>Castro, Dolores</creatorcontrib><creatorcontrib>Borrego, Juan J</creatorcontrib><creatorcontrib>Labella, Alejandro M</creatorcontrib><title>Immune response of DNA vaccinated-gilthead seabream ( Sparus aurata ) against LCDV-Sa infection: relevance of the inflammatory process</title><title>Frontiers in immunology</title><addtitle>Front Immunol</addtitle><description>Lymphocystis disease is one of the main viral pathologies affecting cultured gilthead seabream (
) in the Mediterranean region. Recently, we have developed a DNA vaccine based on the major capsid protein (MCP) of the
(LCDV-Sa). The immune response triggered by either LCDV-Sa infection or vaccination have been previously studied and seem to be highly related to the modulation of the inflammatory and the IFN response. However, a comprehensive evaluation of immune-related gene expression in vaccinated fish after viral infection to identify immunogenes involved in vaccine-induced protection have not been carried out to date. The present study aimed to fulfill this objective by analyzing samples of head-kidney, spleen, intestine, and caudal fin from fish using an OpenArray
platform containing targets related to the immune response of gilthead seabream. The results obtained showed an increase of deregulated genes in the hematopoietic organs between vaccinated and non-vaccinated fish. However, in the intestine and fin, the results showed the opposite trend. The global effect of fish vaccination was a significant decrease (
<0.05) of viral replication in groups of fish previously vaccinated, and the expression of the following immune genes related to viral recognition (
), humoral and cellular response (
and
), inflammation (
,
,
, and
), antiviral response (
,
,
,
), cell-mediated cytotoxicity (
), and apoptosis (
). The exclusive modulation of the immune response provoked by the vaccination seems to control the progression of the infection in the experimentally challenged gilthead seabream.</description><subject>Animals</subject><subject>DNA</subject><subject>DNA Virus Infections</subject><subject>DNA-vaccine</subject><subject>gilthead seabream</subject><subject>Immunity</subject><subject>Immunology</subject><subject>inflammation</subject><subject>innate immune response</subject><subject>Iridoviridae - physiology</subject><subject>LCDV-Sa</subject><subject>OpenArray</subject><subject>Sea Bream</subject><issn>1664-3224</issn><issn>1664-3224</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>DOA</sourceid><recordid>eNpVkk1v1DAQhiMEolXpH-CAfCyHLLEde20uqNrysdIKDgWu1tiZbF0lcbCdlfoH-N1kP6jauXjkmXnmlf0WxVtaLThX-kPr-35asIrxBWWV1ky-KM6plHXJGatfPsnPisuU7qs5as05F6-LM77ktaxqcV78Xc-YAUnENIYhIQktufl-TXbgnB8gY1NufZfvEBqSEGxE6MkVuR0hTonAFCEDeU9gC35ImWxWN7_LWyB-aNFlH4aPM7nDHQzugJ5B-1oHfQ85xAcyxuAwpTfFqxa6hJen86L49eXzz9W3cvPj63p1vSldLXUuEVFKS9WyxUa5xjLJBQWtnLUKLEqtsIHaikYqlGC1U1RoJSQDqaCtW35RrI_cJsC9GaPvIT6YAN4cLkLcGojZuw4Nq1DIlmpw-4eDClAqJoWzEjWnIGbWpyNrnGyPjcMhR-ieQZ9XBn9ntmFnaMWWs6p6JlydCDH8mTBl0_vksOtgwDAlwxRTS6ErpudWdmx1MaQUsX3cQyuzN4Q5GMLsDWFOhpiH3j1V-Djy__v5P7TktPM</recordid><startdate>20230606</startdate><enddate>20230606</enddate><creator>Leiva-Rebollo, Rocio</creator><creator>Gémez-Mata, Juan</creator><creator>Castro, Dolores</creator><creator>Borrego, Juan J</creator><creator>Labella, Alejandro M</creator><general>Frontiers Media S.A</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20230606</creationdate><title>Immune response of DNA vaccinated-gilthead seabream ( Sparus aurata ) against LCDV-Sa infection: relevance of the inflammatory process</title><author>Leiva-Rebollo, Rocio ; Gémez-Mata, Juan ; Castro, Dolores ; Borrego, Juan J ; Labella, Alejandro M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c469t-eee66b187fed8cdb26351a98cbb8abe698eda4b5d68e6ab9c81598562a68af4f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Animals</topic><topic>DNA</topic><topic>DNA Virus Infections</topic><topic>DNA-vaccine</topic><topic>gilthead seabream</topic><topic>Immunity</topic><topic>Immunology</topic><topic>inflammation</topic><topic>innate immune response</topic><topic>Iridoviridae - physiology</topic><topic>LCDV-Sa</topic><topic>OpenArray</topic><topic>Sea Bream</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Leiva-Rebollo, Rocio</creatorcontrib><creatorcontrib>Gémez-Mata, Juan</creatorcontrib><creatorcontrib>Castro, Dolores</creatorcontrib><creatorcontrib>Borrego, Juan J</creatorcontrib><creatorcontrib>Labella, Alejandro M</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>Frontiers in immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Leiva-Rebollo, Rocio</au><au>Gémez-Mata, Juan</au><au>Castro, Dolores</au><au>Borrego, Juan J</au><au>Labella, Alejandro M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Immune response of DNA vaccinated-gilthead seabream ( Sparus aurata ) against LCDV-Sa infection: relevance of the inflammatory process</atitle><jtitle>Frontiers in immunology</jtitle><addtitle>Front Immunol</addtitle><date>2023-06-06</date><risdate>2023</risdate><volume>14</volume><spage>1209926</spage><epage>1209926</epage><pages>1209926-1209926</pages><issn>1664-3224</issn><eissn>1664-3224</eissn><abstract>Lymphocystis disease is one of the main viral pathologies affecting cultured gilthead seabream (
) in the Mediterranean region. Recently, we have developed a DNA vaccine based on the major capsid protein (MCP) of the
(LCDV-Sa). The immune response triggered by either LCDV-Sa infection or vaccination have been previously studied and seem to be highly related to the modulation of the inflammatory and the IFN response. However, a comprehensive evaluation of immune-related gene expression in vaccinated fish after viral infection to identify immunogenes involved in vaccine-induced protection have not been carried out to date. The present study aimed to fulfill this objective by analyzing samples of head-kidney, spleen, intestine, and caudal fin from fish using an OpenArray
platform containing targets related to the immune response of gilthead seabream. The results obtained showed an increase of deregulated genes in the hematopoietic organs between vaccinated and non-vaccinated fish. However, in the intestine and fin, the results showed the opposite trend. The global effect of fish vaccination was a significant decrease (
<0.05) of viral replication in groups of fish previously vaccinated, and the expression of the following immune genes related to viral recognition (
), humoral and cellular response (
and
), inflammation (
,
,
, and
), antiviral response (
,
,
,
), cell-mediated cytotoxicity (
), and apoptosis (
). The exclusive modulation of the immune response provoked by the vaccination seems to control the progression of the infection in the experimentally challenged gilthead seabream.</abstract><cop>Switzerland</cop><pub>Frontiers Media S.A</pub><pmid>37346045</pmid><doi>10.3389/fimmu.2023.1209926</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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| source | PubMed Central |
| subjects | Animals DNA DNA Virus Infections DNA-vaccine gilthead seabream Immunity Immunology inflammation innate immune response Iridoviridae - physiology LCDV-Sa OpenArray Sea Bream |
| title | Immune response of DNA vaccinated-gilthead seabream ( Sparus aurata ) against LCDV-Sa infection: relevance of the inflammatory process |
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