Innovative Surface Plasmon Resonance Aptasensor for Detecting Cocaine in Human Urine

This study describes the development of an optical-based surface plasmon resonance (SPR) aptasensor for the detection of cocaine. The aptasensor was prepared by first attaching gold nanoparticles to a clean SPR chip surface, followed by the addition of an aptamer to create a modified surface. This s...

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Bibliographic Details
Published in:Analytica 2024-11, Vol.5 (4), p.587-598
Main Authors: Keklik Şah, Gül, Isbir Turan, Aybüke A.
Format: Article
Language:English
Subjects:
aptamer
Biomarkers
Biosensors
Chromatography
Cocaine
Contact angle
Cost analysis
Environmental monitoring
Enzymes
Gold
gold nanoparticle
Homogeneity
Immunoassay
Isotherms
Laboratories
Methods
Nanoparticles
Sensors
surface characterization
Surface plasmon resonance
Surface roughness
synthetic urine
Urine
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Summary:This study describes the development of an optical-based surface plasmon resonance (SPR) aptasensor for the detection of cocaine. The aptasensor was prepared by first attaching gold nanoparticles to a clean SPR chip surface, followed by the addition of an aptamer to create a modified surface. This surface was characterized using contact angle and atomic force microscopy, revealing surface roughness values of 0.28 nm and 28.12 nm for the blank and modified surfaces, respectively. The detection of cocaine was carried out in the concentration range of 1 ng/mL to 1000 ng/mL, with a detection time of approximately 8 min and a cocaine limit of detection (LOD) of 0.43 ng/mL. Repeatability studies were conducted, and the stability of the signal response was examined at a concentration of 200 ng/mL. Adsorption isotherm models, including Scatchard, Langmuir, and Freundlich models, were calculated to assess the surface homogeneity of the SPR aptasensor chip, with the results indicating compatibility with the Langmuir isotherm model.
ISSN:2673-4532
2673-4532
DOI:10.3390/analytica5040039