Salient Findings on Host Range, Resistance Screening, and Molecular Studies on Sterility Mosaic Disease of Pigeonpea Induced by Pigeonpea sterility mosaic viruses ( PPSMV-I and PPSMV-II )

• Published in: Frontiers in microbiology 2022-04, Vol.13, p.838047-838047
• Main Authors: Sayiprathap, B R, Patibanda, A K, Prasanna Kumari, V, Jayalalitha, K, Ramappa, H K, Rajeswari, E, Karthiba, L, Saratbabu, K, Sharma, Mamta, Sudini, H K
• Format: Article
• Language: English
• Subjects: Cajanus cajan; host-plant resistance (HPR); host-range; Microbiology; PPSMV-I; PPSMV-II; SMD
• ISSN: 1664-302X; 1664-302X
• DOI: 10.3389/fmicb.2022.838047

Two distinct emaraviruses, ( ) and ( ) were found to be associated with sterility mosaic disease (SMD) of pigeonpea [ (L.) Millsp.]. The host range of both these viruses and their vector are narrow, confined to identified through mechanical transmission, and to cvs. Top Crop, Kintoki, and Bountiful (F: Fabaceae) through mite transmission. A weed host (F: Euphorbiaceae) was also infected and tested positive for both the viruses in RT-PCR. Among the wild species tested, accessions 15661, 15668, and 15671, and accessions 15683, 15686, and 15922 were infected by both the viruses and mite vector suggesting possible sources of SMD inoculum. Though accession 15666 of , 15696 of , and 15639 of were infected by both the viruses, no mite infestation was observed on them. Phylogenetic analysis of nucleotide sequences of RNA-1 and RNA-2 of and isolates in southern India revealed significant divergence especially , which is closely related to the ( ) than . In multilocation testing of pigeonpea genotypes for their broad-based resistance to SMD for two consecutive years, genotypes ICPL-16086 and ICPL-16087 showed resistance reaction (