Molecular identification of some wild Nigerian mushrooms using internal transcribed spacer: polymerase chain reaction

Identification of fungal species based on morphological characteristics is tedious, complex, prone to errors, and thus cannot be completely relied upon. In this study, internal transcribed spacers (ITS 1 and 4)—polymerase chain reaction was employed to amplify DNA of 19 mushroom isolates collected a...

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Bibliographic Details
Published in:AMB Express 2018-09, Vol.8 (1), p.148-9, Article 148
Main Authors: Adeniyi, Mobolaji, Titilawo, Yinka, Oluduro, Anthonia, Odeyemi, Olu, Nakin, Motebang, Okoh, Anthony Ifeanyi
Format: Article
Language:English
Subjects:
Biomedical and Life Sciences
Biotechnology
Deoxyribonucleic acid
DNA
ENPOST
Food security
Life Sciences
Microbial Genetics and Genomics
Microbiology
Molecular identification
Mushrooms
Nigeria
Original
Original Article
Phylogeny
Physical characteristics
Pleurotus pulmonarius
Polymerase chain reaction
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Summary:Identification of fungal species based on morphological characteristics is tedious, complex, prone to errors, and thus cannot be completely relied upon. In this study, internal transcribed spacers (ITS 1 and 4)—polymerase chain reaction was employed to amplify DNA of 19 mushroom isolates collected at Environmental Pollution Science and Technology farm, Ilesa, Southwest Nigeria. The PCR amplification of ITS1 and 4 of the mushrooms isolates yielded approximately 850 bp. Amplicons obtained were sequenced and identified using BLASTn in the NCBI. The BLASTn results revealed that Termitomyces aurantiacus (3), Tricholoma matsutake (8), Tricholoma robustum (2), P . ostreatus (4), Schizophyllum commune (1) and Pleurotus pulmonarius (1) were fully represented. Only Tricholoma matsutake (KT273371), Pleurotus pulmonarius (KY962469) and Tricholoma matsutake (AF438605) had 100% similarity with reference strain. However, the phylogenetic analysis of the isolates showed low genetic relatedness with reference strains. This study revealed the novelty of the mushroom strains and thus advocating the need for strict conservation measures and further investigations on their potential benefits to mankind.
ISSN:2191-0855
2191-0855
DOI:10.1186/s13568-018-0661-9