UPLC-HRMS-Based Plasma Metabolomic Profiling of Novel Biomarkers by Treatment with KDZI in Cerebral Ischemia Reperfusion Rats

• Published in: Molecules (Basel, Switzerland) Switzerland), 2018-05, Vol.23 (6), p.1315
• Main Authors: Wang, Chunguo, Liu, Chenyue, Wang, Min, Ma, Quantao, Li, Yaqi, Wang, Ting, Zhao, Baosheng
• Format: Article
• Language: English
• Subjects: Acetic acid; Angina; Angina pectoris; Animals; Arachidonic acid; Biomarkers; Brain Ischemia - blood; Brain Ischemia - drug therapy; Brain Ischemia - pathology; Cardiovascular disease; Cardiovascular diseases; Cerebral blood flow; Cerebral infarction; Chromatography, High Pressure Liquid; Coronary artery disease; Disease Models, Animal; Drugs, Chinese Herbal - administration & dosage; Heart diseases; Herbal medicine; Infarction; Injection; Ischemia; Kudiezi injection; Leucine; Lipid metabolism; Liquid chromatography; Mass Spectrometry; Mass spectroscopy; Medicine, Chinese Traditional; Metabolic Networks and Pathways; Metabolism; Metabolome; Metabolomics; Metabolomics - methods; middle cerebral artery occlusion and reperfusion; Occlusion; Pharmacology; Phenylalanine; Rats; Reperfusion; Reperfusion Injury - blood; Reperfusion Injury - drug therapy; Reperfusion Injury - pathology; Tryptophan; UPLC-HRMS; Valine
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules23061315

Kudiezi injection (KDZI), also known as Diemailing injection, is a traditional Chinese medicine injection of the composite plant Hance (also known as Kudiezi), and has been widely used to treat coronary heart disease, angina pectoris, and cerebral infarction, but its pharmacological mechanisms remain unclear. This study is designed to explore the effects of KDZI on middle cerebral artery occlusion and reperfusion (MCAO/R) rats, and to identify metabolic features of cerebral ischemia reperfusion by using a nontargeted metabolic profiling method based on ultra-performance liquid chromatography-high resolution mass spectrometry (UPLC-HRMS). In this process, 32 potential biomarkers were found in plasma. KDZI significantly upregulated the levels of taurochenodesoxycholic acid, leucine, l-phenylalanine, l-tryptophan, arachidonic acid (ARA), and phosphatidyl ethanolamines (PE), phosphatidyl cholines (PC) and downregulated the levels of l-valine and 5-hydroxyindole-3-acetic acid (5-HIAA) in plasma. The results indicated that the mechanisms of KDZI on MCAO/R were related to the mechanisms of amino acid and lipid metabolism.