Immunomodulatory potential of Sarcophaga argyostoma larval hemolymph as a natural alternative to berenil in treating Trypanosoma evansi in vivo

This study compared effects of diminazene aceturate (berenil), commonly used to treat domestic animals infected with Trypanosoma evansi, with the hemolymph of Sarcophaga argyostoma larva. The hemolymph may be acting as a possible natural alternative to berenil, based on immunomodulation mediated inf...

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Published in:Scientific reports 2024-03, Vol.14 (1), p.6972-6972, Article 6972
Main Authors: Sadek, Al-Shaimaa M., Farghaly, Doaa S., Kadada, Hala, Mashaal, Alya
Format: Article
Language:English
Subjects:
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Amino acids
Cancer
Carbohydrates
Chromatography
Domestic animals
Entomology
Fatty acids
Folk medicine
Girls
Hemolymph
Humanities and Social Sciences
Immunomodulation
Inflammation
Insects
Laboratory animals
multidisciplinary
Natural products
Peptides
Sarcophaga
Science
Science (multidisciplinary)
Serum levels
Trypanosoma evansi
Tumor necrosis factor-α
Zoology
γ-Interferon
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Summary:This study compared effects of diminazene aceturate (berenil), commonly used to treat domestic animals infected with Trypanosoma evansi, with the hemolymph of Sarcophaga argyostoma larva. The hemolymph may be acting as a possible natural alternative to berenil, based on immunomodulation mediated inflammatory response. Inflammatory mediators and histopathological changes in liver, kidney, and spleen of albino mice experimentally infected with T. evansi were studied. Mice were divided into five groups: G1, uninfected, untreated (negative control); G2, T. evansi infected (positive control); G3, infected and treated with berenil; G4, infected and treated with hemolymph; G5, infected and treated with hemolymph 3 days before infection (prophylactic group). Animals in (G4) and (G5) exhibited a significant overall reduction in serum levels of IFN-γ. However, the reduction in TNF-α and IL-6 levels was more limited compared to (G2) and (G3). Notably, an elevation in IL-10 levels was observed compared to animals in other groups. Furthermore, the groups treated with hemolymph demonstrated an alleviation of T. evansi infection in contrast to the other groups. This study highlights that the administration of Sarcophaga argyostoma larval hemolymph at a dosage of 0.5 ml/kg significantly inhibited T. evansi organisms in vivo, showcasing a pronounced trypanocidal effect.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-024-57113-y