Prevalence and phenotypic pattern of antibiotic resistance of Acinetobacter baumannii isolated from different types of raw meat samples in Isfahan, Iran

• Published in: Veterinary medicine and science 2020-02, Vol.6 (1), p.147-153
• Main Authors: Askari, Neda, Momtaz, Hassan, Tajbakhsh, Elahe
• Format: Article
• Language: English
• Subjects: Acinetobacter baumannii; Acinetobacter baumannii - drug effects; Acinetobacter baumannii - genetics; Acinetobacter baumannii - physiology; Acinetobacter Infections - epidemiology; Acinetobacter Infections - microbiology; Acinetobacter Infections - veterinary; Animals; Anti-Bacterial Agents - pharmacology; Antibiotic resistance; Antibiotics; Antimicrobial agents; Azithromycin; Bacteria; Camelus; Cattle; Cattle Diseases - epidemiology; Cattle Diseases - microbiology; Chickens; Chloramphenicol; Ciprofloxacin; Contaminants; Deoxyribonucleic acid; DNA; Drug resistance; Drug Resistance, Multiple, Bacterial; Epidemics; Erythromycin; Food; Food contamination; Gentamicin; Goat Diseases - epidemiology; Goat Diseases - microbiology; Goats; Hospitals; Imipenem; Infections; Iran; Iran - epidemiology; LAMP assay; Meat; Meat - microbiology; Motility; Original; Oxidation; Phenotype; Poultry Diseases - epidemiology; Poultry Diseases - microbiology; Prevalence; Raw meat; Rifampin; Sheep; Sheep Diseases - epidemiology; Sheep Diseases - microbiology; Sheep, Domestic; Statistical analysis; Trimethoprim; Turkeys
• ISSN: 2053-1095; 2053-1095
• DOI: 10.1002/vms3.199

Resistant Acinetobacter baumannii isolates are not only known as opportunistic nosocomial bacteria but may also be regarded as emerging bacterial contaminants in foods of animal origins. The present investigation was done to assess the prevalence and antibiotic resistance pattern of A. baumannii isolated from different types of raw meat samples. One hundred and ninety‐four raw meat samples were collected and cultured for A. baumannii isolates. Culture‐positive bacteria were also approved using the loop‐mediated isothermal amplification (LAMP) technique. The disc diffusion method was used for antibiotic susceptibility testing. Out of 194 raw meat samples, 39 (20.10%) were positive for A. baumannii isolates. Ovine raw meat was the most commonly contaminated samples (32.14%). All of the culture‐positive A. baumannii isolates were also approved using the LAMP assay. A. baumannii isolates harboured the highest prevalence of resistance against gentamicin (87.17%), tetracycline (79.48%), erythromycin (74.35%), azithromycin (66.66%), ciprofloxacin (58.97%), trimethoprim/sulphamethoxazole (56.41%) and rifampin (51.28%). The lowest prevalence of resistance was found against imipenem (17.94%) and chloramphenicol (28.20%). Raw bovine, ovine, caprine, camel and poultry meat samples were considered as the important sources of isolates resistant to some of the categories of antimicrobials used to treat infections caused by A. baumannii. Further studies are required to find the exact role of resistant A. baumannii isolates in the dissemination of antibiotic resistance to human population. Acinetobacter baumannii, LAMP‐PCR, Antibiotic resistance, Raw meat, Iran.