Drying Enhances Signal Intensities for Global GC⁻MS Metabolomics

We report here that a straightforward change of the standard derivatization procedure for GC⁻MS metabolomics is leading to a strong increase in metabolite signal intensity. Drying samples between methoxymation and trimethylsilylation significantly increased signals by two- to tenfold in extracts of...

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Bibliographic Details
Published in:Metabolites 2019-04, Vol.9 (4), p.68
Main Authors: Liebeke, Manuel, Puskás, Erik
Format: Article
Language:English
Subjects:
Amino acids
Chromatography
Communication
derivatization
Drying
gas chromatography
Laboratories
Metabolism
Metabolites
Metabolomics
Methods
Plant cells
Plant extracts
Reagents
Reproducibility
sample preparation
silylation
Urine
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Summary:We report here that a straightforward change of the standard derivatization procedure for GC⁻MS metabolomics is leading to a strong increase in metabolite signal intensity. Drying samples between methoxymation and trimethylsilylation significantly increased signals by two- to tenfold in extracts of yeast cells, plant and animal tissue, and human urine. This easy step reduces the cost of sample material and the need for expensive new hardware.
ISSN:2218-1989
2218-1989
DOI:10.3390/metabo9040068