Green Synthesis of Zinc Oxide Nanoparticles (ZnO-NPs) Using Arthrospira platensis (Class: Cyanophyceae) and Evaluation of their Biomedical Activities

• Published in: Nanomaterials (Basel, Switzerland) Switzerland), 2021-01, Vol.11 (1), p.95
• Main Authors: El-Belely, Ehab F, Farag, Mohamed M S, Said, Hanan A, Amin, Abeer S, Azab, Ehab, Gobouri, Adil A, Fouda, Amr
• Format: Article
• Language: English
• Subjects: Algae; Antiinfectives and antibacterials; antimicrobial; Antimicrobial agents; Aquatic microorganisms; Arthrospira platensis; Biomass; Biosynthesis; Crystallography; Cyanobacteria; Cyanophyceae; Cytotoxicity; Deoxyribonucleic acid; DNA; E coli; Enzymes; Escherichia coli; Filtrate; Fourier analysis; Fourier transforms; Gene banks; Hazardous materials; in vitro cytotoxicity; Infrared analysis; Infrared spectroscopy; Metabolites; Minimum inhibitory concentration; Nanoparticles; Nanotechnology; Photoelectron spectroscopy; Photoelectrons; Pseudomonas aeruginosa; Spectroscopy; Spectrum analysis; Staphylococcus aureus; Transmission electron microscopy; X ray photoelectron spectroscopy; X-ray diffraction; Zinc oxide; Zinc oxides; ZnO-NPs
• ISSN: 2079-4991; 2079-4991
• DOI: 10.3390/nano11010095

In this study, zinc oxide nanoparticles (ZnO-NPs) were successfully fabricated through the harnessing of metabolites present in the cell filtrate of a newly isolated and identified microalga (Class: Cyanophyceae). The formed ZnO-NPs were characterized by UV-Vis spectroscopy, Fourier transform infrared (FT-IR) transmission electron microscopy (TEM), energy-dispersive spectroscopy (EDX), X-ray diffraction (XRD), and X-ray photoelectron spectroscopy (XPS). Data showed the efficacy of cyanobacterial metabolites in fabricating spherical, crystallographic ZnO-NPs with a size ≈30.0 to 55.0 nm at a wavelength of 370 nm. Moreover, FT-IR analysis showed varied absorption peaks related to nanoparticle formation. XPS analysis confirms the presence of Zn(II)O at different varied bending energies. Data analyses exhibit that the activities of biosynthesized ZnO-NPs were dose-dependent. Their application as an antimicrobial agent was examined and formed clear zones, 24.1 ± 0.3, 21.1 ± 0.06, 19.1 ± 0.3, 19.9 ± 0.1, and 21.6 ± 0.6 mm, at 200 ppm against , , , , and , respectively, and these activities were reduced as the NPs concentration decreased. The minimum inhibitory concentration (MIC) values were determined as 50 ppm for 25 ppm for , and 12.5 ppm for , , and More interestingly, ZnO-NPs exhibit high in vitro cytotoxic efficacy against cancerous (Caco-2) (IC = 9.95 ppm) as compared with normal (WI38) cell line (IC = 53.34 ppm).