Autoclaving-Triggered Hydrogelation of Chitosan-Gluconic acid Conjugate Aqueous Solution for Wound Healing

Moist wound healing is known to heal wounds faster than dry wound healing. Hydrogel wound dressings are suitable for moist wound healing because of their hyperhydrous structure. Chitosan, a natural polymer, promotes wound healing by stimulating inflammatory cells and releasing bioactive compounds. T...

Full description

Saved in:
Bibliographic Details
Published in:Gels 2023-03, Vol.9 (4), p.280
Main Authors: Yamashita, Yusuke, Ohzuno, Yoshihiro, Saito, Yoichi, Fujiwara, Yukio, Yoshida, Masahiro, Takei, Takayuki
Format: Article
Language:English
Subjects:
Acids
Additives
Aqueous solutions
autoclave
Autoclaving
Biocompatibility
Biological properties
Care and treatment
Chitosan
Conjugates
Crosslinked polymers
Crosslinking
Crystal structure
Crystallization
Gluconic acid
hydrogel
Hydrogels
Melting
Natural polymers
Polymers
Pore size
Sterilization
wound dressing
Wound healing
Wounds and injuries
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c609t-124df55d118c2589f95d8eecc0b85df1cef961d7631edfebd8ba5c45fa442d543
cites cdi_FETCH-LOGICAL-c609t-124df55d118c2589f95d8eecc0b85df1cef961d7631edfebd8ba5c45fa442d543
container_end_page
container_issue 4
container_start_page 280
container_title Gels
container_volume 9
creator Yamashita, Yusuke
Ohzuno, Yoshihiro
Saito, Yoichi
Fujiwara, Yukio
Yoshida, Masahiro
Takei, Takayuki
description Moist wound healing is known to heal wounds faster than dry wound healing. Hydrogel wound dressings are suitable for moist wound healing because of their hyperhydrous structure. Chitosan, a natural polymer, promotes wound healing by stimulating inflammatory cells and releasing bioactive compounds. Therefore, chitosan hydrogel has great potential as a wound dressing. In our previous study, physically crosslinked chitosan hydrogels were successfully prepared solely by freeze-thawing of chitosan-gluconic acid conjugate (CG) aqueous solution without using any toxic additives. Furthermore, the CG hydrogels could be sterilized by autoclaving (steam sterilization). In this study, we showed that autoclaving (121 °C, 20 min) of a CG aqueous solution simultaneously achieved gelation of the solution and sterilization of the hydrogel. Hydrogelation of CG aqueous solution by autoclaving is also physically crosslinking without any toxic additives. Further, we showed that the CG hydrogels retained favorable biological properties of the CG hydrogels prepared by freeze-thawing and subsequent autoclaving. These results indicated that CG hydrogels prepared by autoclaving were promising as wound dressings.
doi_str_mv 10.3390/gels9040280
format article
fullrecord <record><control><sourceid>gale_doaj_</sourceid><recordid>TN_cdi_doaj_primary_oai_doaj_org_article_21a2aee623e54671b49cf23d293b23e7</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A747444958</galeid><doaj_id>oai_doaj_org_article_21a2aee623e54671b49cf23d293b23e7</doaj_id><sourcerecordid>A747444958</sourcerecordid><originalsourceid>FETCH-LOGICAL-c609t-124df55d118c2589f95d8eecc0b85df1cef961d7631edfebd8ba5c45fa442d543</originalsourceid><addsrcrecordid>eNptkt9rFDEQxxdRbKl98l0WfBFka37v5kmOQ9tCwQcrPoZsMtlmySU1u1vof2_urtY7kTwkTL7zGeY7U1VvMbqgVKJPA4RJIoZIh15Up4Ri1JBO4JcH75PqfJpGhBBuOeUYv65OaItLhiSn1bha5mSCfvBxaG6zHwbIYOurR5tTYevZp1gnV6_v_JwmHZvLsJgUvam18bZepzgug56hXv1aIC1T_T2FZZfkUq5_piUWGOhQ8G-qV06HCc6f7rPqx9cvt-ur5ubb5fV6ddMYgeTcYMKs49xi3BnCO-kktx2AMajvuHXYgJMC21ZQDNZBb7tec8O404wRyxk9q673XJv0qO6z3-j8qJL2ahdIeVA6z94EUARrogEEocCZaHHPpHGEWiJpX2JtYX3es-6XfgPWQJyzDkfQ45_o79SQHhRGmLYtE4Xw4YmQU7FomtXGTwZC0HHrlyqDE1KKTmyLvf9HOqYlx-LVTsWpbCX7qxp06cBHl0phs4WqVctaxpjkXVFd_EdVjoWNLwME50v8KOHjPsHkNE0Z3HOTGKntqqmDVSvqd4e-PGv_LBb9DfNAzzA</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2806539794</pqid></control><display><type>article</type><title>Autoclaving-Triggered Hydrogelation of Chitosan-Gluconic acid Conjugate Aqueous Solution for Wound Healing</title><source>PubMed Central Free</source><source>Publicly Available Content Database</source><creator>Yamashita, Yusuke ; Ohzuno, Yoshihiro ; Saito, Yoichi ; Fujiwara, Yukio ; Yoshida, Masahiro ; Takei, Takayuki</creator><creatorcontrib>Yamashita, Yusuke ; Ohzuno, Yoshihiro ; Saito, Yoichi ; Fujiwara, Yukio ; Yoshida, Masahiro ; Takei, Takayuki</creatorcontrib><description>Moist wound healing is known to heal wounds faster than dry wound healing. Hydrogel wound dressings are suitable for moist wound healing because of their hyperhydrous structure. Chitosan, a natural polymer, promotes wound healing by stimulating inflammatory cells and releasing bioactive compounds. Therefore, chitosan hydrogel has great potential as a wound dressing. In our previous study, physically crosslinked chitosan hydrogels were successfully prepared solely by freeze-thawing of chitosan-gluconic acid conjugate (CG) aqueous solution without using any toxic additives. Furthermore, the CG hydrogels could be sterilized by autoclaving (steam sterilization). In this study, we showed that autoclaving (121 °C, 20 min) of a CG aqueous solution simultaneously achieved gelation of the solution and sterilization of the hydrogel. Hydrogelation of CG aqueous solution by autoclaving is also physically crosslinking without any toxic additives. Further, we showed that the CG hydrogels retained favorable biological properties of the CG hydrogels prepared by freeze-thawing and subsequent autoclaving. These results indicated that CG hydrogels prepared by autoclaving were promising as wound dressings.</description><identifier>ISSN: 2310-2861</identifier><identifier>EISSN: 2310-2861</identifier><identifier>DOI: 10.3390/gels9040280</identifier><identifier>PMID: 37102892</identifier><language>eng</language><publisher>Switzerland: MDPI AG</publisher><subject>Acids ; Additives ; Aqueous solutions ; autoclave ; Autoclaving ; Biocompatibility ; Biological properties ; Care and treatment ; Chitosan ; Conjugates ; Crosslinked polymers ; Crosslinking ; Crystal structure ; Crystallization ; Gluconic acid ; hydrogel ; Hydrogels ; Melting ; Natural polymers ; Polymers ; Pore size ; Sterilization ; wound dressing ; Wound healing ; Wounds and injuries</subject><ispartof>Gels, 2023-03, Vol.9 (4), p.280</ispartof><rights>COPYRIGHT 2023 MDPI AG</rights><rights>2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2023 by the authors. 2023</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c609t-124df55d118c2589f95d8eecc0b85df1cef961d7631edfebd8ba5c45fa442d543</citedby><cites>FETCH-LOGICAL-c609t-124df55d118c2589f95d8eecc0b85df1cef961d7631edfebd8ba5c45fa442d543</cites><orcidid>0000-0001-7985-1649 ; 0009-0002-3654-3210</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/2806539794/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/2806539794?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,25753,27924,27925,37012,37013,44590,53791,53793,75126</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/37102892$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yamashita, Yusuke</creatorcontrib><creatorcontrib>Ohzuno, Yoshihiro</creatorcontrib><creatorcontrib>Saito, Yoichi</creatorcontrib><creatorcontrib>Fujiwara, Yukio</creatorcontrib><creatorcontrib>Yoshida, Masahiro</creatorcontrib><creatorcontrib>Takei, Takayuki</creatorcontrib><title>Autoclaving-Triggered Hydrogelation of Chitosan-Gluconic acid Conjugate Aqueous Solution for Wound Healing</title><title>Gels</title><addtitle>Gels</addtitle><description>Moist wound healing is known to heal wounds faster than dry wound healing. Hydrogel wound dressings are suitable for moist wound healing because of their hyperhydrous structure. Chitosan, a natural polymer, promotes wound healing by stimulating inflammatory cells and releasing bioactive compounds. Therefore, chitosan hydrogel has great potential as a wound dressing. In our previous study, physically crosslinked chitosan hydrogels were successfully prepared solely by freeze-thawing of chitosan-gluconic acid conjugate (CG) aqueous solution without using any toxic additives. Furthermore, the CG hydrogels could be sterilized by autoclaving (steam sterilization). In this study, we showed that autoclaving (121 °C, 20 min) of a CG aqueous solution simultaneously achieved gelation of the solution and sterilization of the hydrogel. Hydrogelation of CG aqueous solution by autoclaving is also physically crosslinking without any toxic additives. Further, we showed that the CG hydrogels retained favorable biological properties of the CG hydrogels prepared by freeze-thawing and subsequent autoclaving. These results indicated that CG hydrogels prepared by autoclaving were promising as wound dressings.</description><subject>Acids</subject><subject>Additives</subject><subject>Aqueous solutions</subject><subject>autoclave</subject><subject>Autoclaving</subject><subject>Biocompatibility</subject><subject>Biological properties</subject><subject>Care and treatment</subject><subject>Chitosan</subject><subject>Conjugates</subject><subject>Crosslinked polymers</subject><subject>Crosslinking</subject><subject>Crystal structure</subject><subject>Crystallization</subject><subject>Gluconic acid</subject><subject>hydrogel</subject><subject>Hydrogels</subject><subject>Melting</subject><subject>Natural polymers</subject><subject>Polymers</subject><subject>Pore size</subject><subject>Sterilization</subject><subject>wound dressing</subject><subject>Wound healing</subject><subject>Wounds and injuries</subject><issn>2310-2861</issn><issn>2310-2861</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>PIMPY</sourceid><sourceid>DOA</sourceid><recordid>eNptkt9rFDEQxxdRbKl98l0WfBFka37v5kmOQ9tCwQcrPoZsMtlmySU1u1vof2_urtY7kTwkTL7zGeY7U1VvMbqgVKJPA4RJIoZIh15Up4Ri1JBO4JcH75PqfJpGhBBuOeUYv65OaItLhiSn1bha5mSCfvBxaG6zHwbIYOurR5tTYevZp1gnV6_v_JwmHZvLsJgUvam18bZepzgug56hXv1aIC1T_T2FZZfkUq5_piUWGOhQ8G-qV06HCc6f7rPqx9cvt-ur5ubb5fV6ddMYgeTcYMKs49xi3BnCO-kktx2AMajvuHXYgJMC21ZQDNZBb7tec8O404wRyxk9q673XJv0qO6z3-j8qJL2ahdIeVA6z94EUARrogEEocCZaHHPpHGEWiJpX2JtYX3es-6XfgPWQJyzDkfQ45_o79SQHhRGmLYtE4Xw4YmQU7FomtXGTwZC0HHrlyqDE1KKTmyLvf9HOqYlx-LVTsWpbCX7qxp06cBHl0phs4WqVctaxpjkXVFd_EdVjoWNLwME50v8KOHjPsHkNE0Z3HOTGKntqqmDVSvqd4e-PGv_LBb9DfNAzzA</recordid><startdate>20230329</startdate><enddate>20230329</enddate><creator>Yamashita, Yusuke</creator><creator>Ohzuno, Yoshihiro</creator><creator>Saito, Yoichi</creator><creator>Fujiwara, Yukio</creator><creator>Yoshida, Masahiro</creator><creator>Takei, Takayuki</creator><general>MDPI AG</general><general>MDPI</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FE</scope><scope>8FG</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>HCIFZ</scope><scope>KB.</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0001-7985-1649</orcidid><orcidid>https://orcid.org/0009-0002-3654-3210</orcidid></search><sort><creationdate>20230329</creationdate><title>Autoclaving-Triggered Hydrogelation of Chitosan-Gluconic acid Conjugate Aqueous Solution for Wound Healing</title><author>Yamashita, Yusuke ; Ohzuno, Yoshihiro ; Saito, Yoichi ; Fujiwara, Yukio ; Yoshida, Masahiro ; Takei, Takayuki</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c609t-124df55d118c2589f95d8eecc0b85df1cef961d7631edfebd8ba5c45fa442d543</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Acids</topic><topic>Additives</topic><topic>Aqueous solutions</topic><topic>autoclave</topic><topic>Autoclaving</topic><topic>Biocompatibility</topic><topic>Biological properties</topic><topic>Care and treatment</topic><topic>Chitosan</topic><topic>Conjugates</topic><topic>Crosslinked polymers</topic><topic>Crosslinking</topic><topic>Crystal structure</topic><topic>Crystallization</topic><topic>Gluconic acid</topic><topic>hydrogel</topic><topic>Hydrogels</topic><topic>Melting</topic><topic>Natural polymers</topic><topic>Polymers</topic><topic>Pore size</topic><topic>Sterilization</topic><topic>wound dressing</topic><topic>Wound healing</topic><topic>Wounds and injuries</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yamashita, Yusuke</creatorcontrib><creatorcontrib>Ohzuno, Yoshihiro</creatorcontrib><creatorcontrib>Saito, Yoichi</creatorcontrib><creatorcontrib>Fujiwara, Yukio</creatorcontrib><creatorcontrib>Yoshida, Masahiro</creatorcontrib><creatorcontrib>Takei, Takayuki</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>Materials Science &amp; Engineering Collection</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>SciTech Premium Collection</collection><collection>Materials Science Database</collection><collection>Materials Science Collection</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>Gels</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yamashita, Yusuke</au><au>Ohzuno, Yoshihiro</au><au>Saito, Yoichi</au><au>Fujiwara, Yukio</au><au>Yoshida, Masahiro</au><au>Takei, Takayuki</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Autoclaving-Triggered Hydrogelation of Chitosan-Gluconic acid Conjugate Aqueous Solution for Wound Healing</atitle><jtitle>Gels</jtitle><addtitle>Gels</addtitle><date>2023-03-29</date><risdate>2023</risdate><volume>9</volume><issue>4</issue><spage>280</spage><pages>280-</pages><issn>2310-2861</issn><eissn>2310-2861</eissn><abstract>Moist wound healing is known to heal wounds faster than dry wound healing. Hydrogel wound dressings are suitable for moist wound healing because of their hyperhydrous structure. Chitosan, a natural polymer, promotes wound healing by stimulating inflammatory cells and releasing bioactive compounds. Therefore, chitosan hydrogel has great potential as a wound dressing. In our previous study, physically crosslinked chitosan hydrogels were successfully prepared solely by freeze-thawing of chitosan-gluconic acid conjugate (CG) aqueous solution without using any toxic additives. Furthermore, the CG hydrogels could be sterilized by autoclaving (steam sterilization). In this study, we showed that autoclaving (121 °C, 20 min) of a CG aqueous solution simultaneously achieved gelation of the solution and sterilization of the hydrogel. Hydrogelation of CG aqueous solution by autoclaving is also physically crosslinking without any toxic additives. Further, we showed that the CG hydrogels retained favorable biological properties of the CG hydrogels prepared by freeze-thawing and subsequent autoclaving. These results indicated that CG hydrogels prepared by autoclaving were promising as wound dressings.</abstract><cop>Switzerland</cop><pub>MDPI AG</pub><pmid>37102892</pmid><doi>10.3390/gels9040280</doi><orcidid>https://orcid.org/0000-0001-7985-1649</orcidid><orcidid>https://orcid.org/0009-0002-3654-3210</orcidid><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 2310-2861
ispartof Gels, 2023-03, Vol.9 (4), p.280
issn 2310-2861
2310-2861
language eng
recordid cdi_doaj_primary_oai_doaj_org_article_21a2aee623e54671b49cf23d293b23e7
source PubMed Central Free; Publicly Available Content Database
subjects Acids
Additives
Aqueous solutions
autoclave
Autoclaving
Biocompatibility
Biological properties
Care and treatment
Chitosan
Conjugates
Crosslinked polymers
Crosslinking
Crystal structure
Crystallization
Gluconic acid
hydrogel
Hydrogels
Melting
Natural polymers
Polymers
Pore size
Sterilization
wound dressing
Wound healing
Wounds and injuries
title Autoclaving-Triggered Hydrogelation of Chitosan-Gluconic acid Conjugate Aqueous Solution for Wound Healing
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-04T14%3A34%3A12IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_doaj_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Autoclaving-Triggered%20Hydrogelation%20of%20Chitosan-Gluconic%20acid%20Conjugate%20Aqueous%20Solution%20for%20Wound%20Healing&rft.jtitle=Gels&rft.au=Yamashita,%20Yusuke&rft.date=2023-03-29&rft.volume=9&rft.issue=4&rft.spage=280&rft.pages=280-&rft.issn=2310-2861&rft.eissn=2310-2861&rft_id=info:doi/10.3390/gels9040280&rft_dat=%3Cgale_doaj_%3EA747444958%3C/gale_doaj_%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c609t-124df55d118c2589f95d8eecc0b85df1cef961d7631edfebd8ba5c45fa442d543%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=2806539794&rft_id=info:pmid/37102892&rft_galeid=A747444958&rfr_iscdi=true