Expression analysis of centrin gene in promastigote and amastigote forms of leishmania infantum iranian isolates: a promising target for live attenuated vaccine development against canine leishmaniasis

Leishmania parasites express various essential proteins in different growth phases (logarithmic/stationary) and forms (promastigote/amastigote). Targeting the genes encoding such proteins paves the way for controlling these parasites. Centrin is an essential gene, which its protein product seems to...

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Published in:BMC veterinary research 2021-04, Vol.17 (1), p.162-162, Article 162
Main Authors: Abbaszadeh Afshar, Mohammad Javad, Elikaee, Samira, Saberi, Reza, Mohtasebi, Sina, Mohebali, Mehdi
Format: Article
Language:English
Subjects:
Amastigotes
Canine leishmaniasis
Cell culture
Cell division
Centrin gene
Control
Diseases
Dogs
Gene expression
genes
Genetic aspects
Intracellular
Iran
Leishmania
Leishmania infantum
Leishmaniasis
live vaccines
Parasite vaccines
Parasites
Parasitic diseases
Parasitological research
Phosphoproteins
Polymerase chain reaction
Promastigotes
Proteins
Real‐time RT-PCR
Restriction fragment length polymorphism
Reverse transcription
Statistical analysis
Vaccine development
Vaccines
Virulence
Zoonoses
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description Leishmania parasites express various essential proteins in different growth phases (logarithmic/stationary) and forms (promastigote/amastigote). Targeting the genes encoding such proteins paves the way for controlling these parasites. Centrin is an essential gene, which its protein product seems to be vital for the proliferation of Leishmania parasites. Herein, this study was contrived to analyze the expression level of the centrin gene in different growth phases and forms of Leishmania infantum (L. infantum) parasites isolated from various endemic areas of canine leishmaniasis (CanL) in Iran. All three collected isolates were identified as L. infantum using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Real-time reverse transcription (RT)-PCR revealed a statistically significant up-regulation (3.13-fold) in the logarithmic phase promastigotes compared to stationary ones (p  0.05). Centrin gene in Iranian isolates of L. infantum is more expressed in exponential than stationary phases and seems to be considered as a promising target in the development of a genetically modified live attenuated vaccine for CanL control.
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Also, our finding revealed a slight up-regulation of the centrin gene (1.22-fold) in the intracellular amastigotes compared to logarithmic phase promastigotes, which was found statistically non-significant (p &gt; 0.05). Centrin gene in Iranian isolates of L. infantum is more expressed in exponential than stationary phases and seems to be considered as a promising target in the development of a genetically modified live attenuated vaccine for CanL control.</abstract><cop>England</cop><pub>BioMed Central Ltd</pub><pmid>33853591</pmid><doi>10.1186/s12917-021-02816-x</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record>
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1746-6148
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source Publicly Available Content Database; PubMed Central
subjects Amastigotes
Canine leishmaniasis
Cell culture
Cell division
Centrin gene
Control
Diseases
Dogs
Gene expression
genes
Genetic aspects
Intracellular
Iran
Leishmania
Leishmania infantum
Leishmaniasis
live vaccines
Parasite vaccines
Parasites
Parasitic diseases
Parasitological research
Phosphoproteins
Polymerase chain reaction
Promastigotes
Proteins
Real‐time RT-PCR
Restriction fragment length polymorphism
Reverse transcription
Statistical analysis
Vaccine development
Vaccines
Virulence
Zoonoses
title Expression analysis of centrin gene in promastigote and amastigote forms of leishmania infantum iranian isolates: a promising target for live attenuated vaccine development against canine leishmaniasis
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