Molecular Signatures Integral to Natural Reprogramming in the Pigment Epithelium Cells after Retinal Detachment in Pleurodeles waltl

The reprogramming of retinal pigment epithelium (RPE) cells into retinal cells (transdifferentiation) lies in the bases of retinal regeneration in several Urodela. The identification of the key genes involved in this process helps with looking for approaches to the prevention and treatment of RPE-re...

Full description

Saved in:
Bibliographic Details
Published in:International journal of molecular sciences 2023-12, Vol.24 (23), p.16940
Main Authors: Markitantova, Yuliya, Fokin, Alexander, Boguslavsky, Dmitry, Simirskii, Vladimir, Kulikov, Aleksey
Format: Article
Language:English
Subjects:
adult newt
Animals
dedifferentiation
Epithelium
Gene expression
Humans
Morphology
natural RPE reprogramming
Pleurodeles
regeneration
Retina
Retina - metabolism
Retinal detachment
Retinal Detachment - genetics
Retinal Detachment - metabolism
retinal pigment epithelium (RPE)
Retinal Pigment Epithelium - metabolism
Transcriptome
Urodela
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The reprogramming of retinal pigment epithelium (RPE) cells into retinal cells (transdifferentiation) lies in the bases of retinal regeneration in several Urodela. The identification of the key genes involved in this process helps with looking for approaches to the prevention and treatment of RPE-related degenerative diseases of the human retina. The purpose of our study was to examine the transcriptome changes at initial stages of RPE cell reprogramming in adult newt . RPE was isolated from the eye samples of day 0, 4, and 7 after experimental surgical detachment of the neural retina and was used for a de novo transcriptome assembly through the RNA-Seq method. A total of 1019 transcripts corresponding to the differently expressed genes have been revealed in silico: the 83 increased the expression at an early stage, and 168 increased the expression at a late stage of RPE reprogramming. We have identified up-regulation of classical early response genes, chaperones and co-chaperones, genes involved in the regulation of protein biosynthesis, suppressors of oncogenes, and EMT-related genes. We revealed the growth in the proportion of down-regulated ribosomal and translation-associated genes. Our findings contribute to revealing the molecular mechanism of RPE reprogramming in Urodela.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms242316940