Bioactive Properties of a Novel Antibacterial Dye Obtained from Laccase-Mediated Oxidation of 8-Anilino-1-naphthalenesulfonic Acid

• Published in: Molecules (Basel, Switzerland) Switzerland), 2022-01, Vol.27 (2), p.487
• Main Authors: Polak, Jolanta, Grąz, Marcin, Wlizło, Kamila, Szałapata, Katarzyna, Kapral-Piotrowska, Justyna, Paduch, Roman, Jarosz-Wilkołazka, Anna
• Format: Article
• Language: English
• Subjects: Adult; Aged; Aliivibrio fischeri - drug effects; Anilino Naphthalenesulfonates - chemistry; Anilino Naphthalenesulfonates - metabolism; Anti-Bacterial Agents - biosynthesis; Anti-Bacterial Agents - chemistry; Anti-Bacterial Agents - pharmacology; Anti-Bacterial Agents - toxicity; Antibacterial materials; Antimicrobial agents; Antioxidants; Antioxidants - chemistry; Antioxidants - metabolism; Antioxidants - pharmacology; Antioxidants - toxicity; bioactive compounds; Biocatalysis; Biocompatibility; Biosynthesis; Cell Line; Chemical compounds; Colon - drug effects; Coloring Agents - chemistry; Coloring Agents - metabolism; Coloring Agents - pharmacology; Coloring Agents - toxicity; Cytotoxicity; Dyeing; Dyes; Epithelial Cells - drug effects; Female; Fibers; Fibroblasts - drug effects; Fungi - enzymology; Healthy Volunteers; Humans; Hydrocarbons; Hypersensitivity; Laccase; Laccase - chemistry; Laccase - metabolism; Male; Middle Aged; Oxidation; Oxidation-Reduction; Proteins; Skin - drug effects; Solubilization; Solvents; Staphylococcus aureus - drug effects; Staphylococcus epidermidis - drug effects; Substrates; Toxicity
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules27020487

Fungal laccase obtained from a strain was used as an effective biocatalyst for the transformation of 8-anilino-1-naphthalenesulfonic acid into a green-coloured antibacterial compound, which can be considered as both an antimicrobial agent and a textile dye, simultaneously. The process of biosynthesis was performed in buffered solutions containing methanol as a co-solvent, allowing better solubilisation of substrate. The transformation process was optimised in terms of the buffer pH value, laccase activity, and concentrations of the substrate and co-solvent. The crude product obtained exhibited low cytotoxicity, antibacterial properties against and , and antioxidant properties. Moreover, the synthesised green-coloured compound proved non-allergenic and demonstrated a high efficiency of dyeing wool fibres.