Chromosome-level genome assembly of Asian yellow pond turtle (Mauremys mutica) with temperature-dependent sex determination system

Knowledge of sex determination has important implications in physiology, ecology and genetics, but the evolutionary mechanisms of sex determination systems in turtles have not been fully elucidated, due to a lack of reference genomes. Here, we generate a high-quality genome assembly of Asian yellow...

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Published in:Scientific reports 2022-05, Vol.12 (1), p.7905-7905, Article 7905
Main Authors: Liu, Xiaoli, Wang, Yakun, Yuan, Ju, Liu, Fang, Hong, Xiaoyou, Yu, Lingyun, Chen, Chen, Li, Wei, Ni, Wei, Liu, Haiyang, Zhao, Jian, Wei, Chengqing, Chen, Haigang, Liu, Yihui, Zhu, Xinping
Format: Article
Language:English
Subjects:
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631/337
Animals
Calcium signalling
Chromatin
Chromosomes
Conformation
Genetics
Genome Size
Genomes
Haplotypes
Humanities and Social Sciences
Mauremys mutica
multidisciplinary
Phylogeny
Ponds
Science
Science (multidisciplinary)
Sequence Analysis, DNA
Sex determination
Signal transduction
Temperature
Turtles - genetics
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Summary:Knowledge of sex determination has important implications in physiology, ecology and genetics, but the evolutionary mechanisms of sex determination systems in turtles have not been fully elucidated, due to a lack of reference genomes. Here, we generate a high-quality genome assembly of Asian yellow pond turtle ( Mauremys mutica ) using continuous long-read (PacBio platform), Illumina, and high-throughput chromatin conformation capture (Hi-C) technologies. The M . mutica haplotype has a genome size of 2.23 Gb with a contig N50 of 8.53 Mb and scaffold N50 of 141.98 Mb. 99.98% sequences of the total assembly are anchored to 26 pseudochromosomes. Comparative genomics analysis indicated that the lizard-snake-tuatara clade diverged from the bird-crocodilian-turtle clade at approximately 267.0–312.3 Mya. Intriguingly, positive selected genes are mostly enriched in the calcium signaling pathway and neuroactive ligand-receptor interaction, which are involved in the process of temperature-dependent sex determination. These findings provide important evolutionary insights into temperature-dependent sex determination system.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-022-12054-2