Study of saiga horn using high-performance liquid chromatography with mass spectrometry

The saiga horns have been investigated the using of modern analytic methods. High-performance liquid chromatography (HPLC) with mass-spectrometric (MS and MS/MS) detection and polyacrylamide gel electrophoresis (PAGE) were used. It could be concluded that basic proteins of the saiga horns are kerati...

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Bibliographic Details
Published in:TheScientificWorld 2012-01, Vol.2012 (2012), p.1-8
Main Authors: Mikulíková, Kateřina, Romanov, Oleg, Miksik, Ivan, Eckhardt, Adam, Pataridis, Statis, Sedláková, Pavla
Format: Article
Language:English
Subjects:
Amino acids
Animals
Antelopes - metabolism
Antlers
Chemical engineering
Chemical engineering research
Chemical properties
Chromatography, High Pressure Liquid - methods
Collagen
Collagen (type I)
Collagen (type II)
Deoxyribonucleic acid
DNA
Electrophoresis
Fourier transforms
Gel electrophoresis
Hair
High performance liquid chromatography
Horns
Horns - chemistry
Identification and classification
Keratin
Liquid chromatography
Mass spectrometry
Mass Spectrometry - methods
Mass spectroscopy
Methods
Proteins
Saiga tatarica
Scientific imaging
Sheep
Veterinary medicine
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Summary:The saiga horns have been investigated the using of modern analytic methods. High-performance liquid chromatography (HPLC) with mass-spectrometric (MS and MS/MS) detection and polyacrylamide gel electrophoresis (PAGE) were used. It could be concluded that basic proteins of the saiga horns are keratins and collagen. The basic representation protein in all samples is keratin type I microfibrillar (from sheep), keratin type II microfibrillar (from sheep), collagen type I (α1) (from bovine) and collagen type I (α2) (from bovine). Free amino acids we determined in all samples are nontreated by enzyme.
ISSN:2356-6140
1537-744X
1537-744X
DOI:10.1100/2012/759604