Transcriptomic Analysis, Motility and Biofilm Formation Characteristics of Salmonella typhimurium Exposed to Benzyl Isothiocyanate Treatment

( ) is a common foodborne pathogen that not only causes diseases and contaminates food, but also causes considerable economic losses. Therefore, it is necessary to find effective and feasible methods to control . In this study, changes in after treatment with benzyl isothiocyanate (BITC) were detect...

Full description

Saved in:
Bibliographic Details
Published in:International journal of molecular sciences 2020-02, Vol.21 (3), p.1025
Main Authors: Niu, Tong-Xin, Wang, Xiao-Ning, Wu, Hong-Yan, Bi, Jing-Ran, Hao, Hong-Shun, Hou, Hong-Man, Zhang, Gong-Liang
Format: Article
Language:English
Subjects:
Antibacterial activity
Antiinfectives and antibacterials
Antimicrobial agents
Bacteria
benzyl isothiocyanate
biofilm
Biofilms
Control methods
Economic impact
Food contamination
Food safety
Foodborne pathogens
Gene expression
Genes
Infections
Isothiocyanate
Metabolism
Motility
Ontology
Pathogens
Polymerase chain reaction
Proteins
Salmonella
Salmonella Typhimurium
Swimming
transcriptome
Virulence
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:( ) is a common foodborne pathogen that not only causes diseases and contaminates food, but also causes considerable economic losses. Therefore, it is necessary to find effective and feasible methods to control . In this study, changes in after treatment with benzyl isothiocyanate (BITC) were detected by transcriptomics to explore the antibacterial effect of BITC at subinhibitory concentration. The results showed that, in contrast to the control group (SC), the BITC-treated group (SQ_BITC) had 197 differentially expressed genes (DEGs), of which 115 were downregulated and 82 were upregulated. We screened out eight significantly downregulated virulence-related genes and verified gene expression by quantitative Real-time Polymerase Chain Reaction (qRT-PCR). We also selected motility and biofilm formation to observe the effects of BITC on the other virulence related factors of . The results showed that both swimming and swarming were significantly inhibited. BITC also had a significant inhibitory effect on biofilm formation, and showed an effect on bacterial morphology. These results will be helpful for understanding the mechanism of the antibacterial action of BITC against and other foodborne pathogens.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms21031025