Monkeypox virus genome sequence from an imported human case in Colombia

Introduction: Monkeypox virus (MPXV) is an enveloped double-stranded DNA virus with a genome of approximately 197.209 bp. The current classification divides MPXV into three clades: Clade I (Central African or Congo Basin clade) and clades IIa and IIb (West African clades).Objective: To report the co...

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Bibliographic Details
Published in:Biomédica 2022-09, Vol.42 (3), p.541-545
Main Authors: Laiton-Donato, Katherine, Álvarez-Díaz, Diego A., Franco-Muñoz, Carlos, Ruiz-Moreno, Héctor A., Rojas-Estévez, Paola, Prada, Andrés, Rosales, Alicia, Ospina, Martha Lucía, Mercado-Reyes, Marcela
Format: Article
Language:English
Subjects:
Brief Communication
colombia
monkeypox virus
nanopore sequencing
phylogeny
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Summary:Introduction: Monkeypox virus (MPXV) is an enveloped double-stranded DNA virus with a genome of approximately 197.209 bp. The current classification divides MPXV into three clades: Clade I (Central African or Congo Basin clade) and clades IIa and IIb (West African clades).Objective: To report the complete genome and phylogenetic analysis of a human monkeypox case detected in Colombia.Materials and methods: Exudate from vesicular lesions was obtained from a male patient with recent travel history to Spain. A direct genomic approach was implemented in which total DNA from the sample was purified through a column-based method, followed by sequencing on the Nanopore GridION. Reads were aligned against the MPXV reference genome using minimap2 v.2.24 and phylogenetic inference was performed using maximum likelihood estimation.Results: A total of 11.951 reads mapped directly to a reference genome with 96.8% of coverage (190.898 bp).Conclusion: Phylogenetic analysis of the MPXV circulating in Colombia demonstrated its close relationship to clade IIb responsible for the multi-country outbreak in 2022.
ISSN:0120-4157
2590-7379
DOI:10.7705/biomedica.6647