Platelet-rich plasma induces post-natal maturation of immature articular cartilage and correlates with LOXL1 activation

Platelet-rich plasma (PRP) is used to stimulate the repair of acute and chronic cartilage damage even though there is no definitive evidence of how this is achieved. Chondrocytes in injured and diseased situations frequently re-express phenotypic biomarkers of immature cartilage so tissue maturation...

Full description

Saved in:
Bibliographic Details
Published in:Scientific reports 2017-06, Vol.7 (1), p.3699-12, Article 3699
Main Authors: Zhang, Yadan, Morgan, Ben J., Smith, Rachel, Fellows, Christopher R., Thornton, Catherine, Snow, Martyn, Francis, Lewis W., Khan, Ilyas M.
Format: Article
Language:English
Subjects:
14/1
14/63
38
38/39
38/77
64
692/308/575
692/4023/1671/1354
Amino Acid Oxidoreductases - genetics
Amino Acid Oxidoreductases - metabolism
Animals
Atomic force microscopy
Biomarkers
Bromodeoxyuridine
Cartilage (articular)
Cartilage, Articular - cytology
Cartilage, Articular - metabolism
Cattle
Cell density
Cell Differentiation - genetics
Cell Proliferation
Chondrocytes
Chondrogenesis - genetics
Collagen
Comparative studies
Fluorescent Antibody Technique
Gene expression
Gene Expression Regulation
Growth factors
Humanities and Social Sciences
Iodide peroxidase
Lysyl oxidase
Male
Maturation
multidisciplinary
Netrin-1
Platelet-Rich Plasma
Platelets
Proliferating cell nuclear antigen
Science
Science (multidisciplinary)
Structure-function relationships
Transcriptional Activation
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Platelet-rich plasma (PRP) is used to stimulate the repair of acute and chronic cartilage damage even though there is no definitive evidence of how this is achieved. Chondrocytes in injured and diseased situations frequently re-express phenotypic biomarkers of immature cartilage so tissue maturation is a potential pathway for restoration of normal structure and function. We used an in vitro model of growth factor-induced maturation to perform a comparative study in order to determine whether PRP can also induce this specific form of remodeling that is characterised by increased cellular proliferation and tissue stiffness. Gene expression patterns specific for maturation were mimicked in PRP treated cartilage, with chondromodulin, collagen types II/X downregulated, deiodinase II and netrin-1 upregulated. PRP increased cartilage surface cell density 1.5-fold ( P  
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-017-02297-9