Screening of edible mushrooms and extraction by pressurized water (PWE) of 3-hydroxy-3-methyl-glutaryl CoA reductase inhibitors

• Published in: Journal of functional foods 2013-01, Vol.5 (1), p.244-250
• Main Authors: Gil-Ramírez, Alicia, Clavijo, Cristina, Palanisamy, Marimuthu, Ruiz-Rodríguez, Alejandro, Navarro-Rubio, María, Pérez, Margarita, Marín, Francisco R., Reglero, Guillermo, Soler-Rivas, Cristina
• Format: Article
• Language: English
• Subjects: Accelerated solvent extraction (ASE); Cholesterol; HMGCoA reductase; Lentinus edodes; Pleurotus ostreatus
• ISSN: 1756-4646
• DOI: 10.1016/j.jff.2012.10.013

The methanol/water and particularly the water extracts obtained from 26 mushroom species were able to inhibit the 3-hydroxy-3-methyl-glutaryl CoA reductase (HMGCR) activity to different extent (10–76%). Cultivated mushrooms such as Pleurotus sp. and Lentinula edodes were among the strains which showed higher HMGCR inhibitory capacities. Their inhibitory properties were not largely influenced by cultivation parameters, mushroom developmental stage or flush number. The HMGCR inhibitory activity of L. edodes was concentrated in the cap excluding the gills while in Pleurotus ostreatus it was distributed through all the different tissues. A method to obtain aqueous fractions with high HMGCR inhibitory activity was optimized using an accelerated solvent extractor (ASE) by selecting 10.7 MPa and 25 °C as common extraction conditions and 5 cycles of 5 min each for P. ostreatus fruiting bodies and 15 cycles of 5 min for L. edodes suggesting that the potential HMGCR inhibitors are different in the two selected mushrooms.